Icerya purchasi

Transcriptome of Icerya purchasi

"Construction of libraries and sequencing on the Illumina HiSeq2500 were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from one microgram of high quality total RNA. RNAseq libraries were constructed using the TruSeq Stranded RNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq2500 with TruSeq SBS sequencing kits version 4. The libraries were sequenced from both ends of the molecules to a total read length of 100nt from each end. The raw .bcl files were converted into demultiplexed fastq files with Casava 1.8.2 (Illumina)."

{'host': 'Pittosporum tobira', 'breed': 'wildtype', 'tissue': 'whole insect', 'collected_by': 'Nate Hardy', 'geo_loc_name': 'USA: AL, Auburn', 'biosamplemodel': 'Invertebrate', 'collection_date': '2015-02-09', 'isolation_source': 'wild-caught'}

Illumina NovaSeq X paired end sequencing

Illumina sequencing of sample accession SAMEA12110749 for study accession PRJEB53240. This submission includes reads tagged with the sequence CCGGGAAT.

{'gal': 'Sanger Institute', 'sex': 'hermaphrodite and monoecious', 'tolid': 'ihIcePurc11', 'habitat': 'Laboratory line on Pittosporum tobira plant', 'lifestage': 'juvenile', 'broker name': 'Collaborative Open Plant Omics broker account, Earlham Institute, Norwich', 'external id': 'SAMEA12110749', 'sample name': '61b9bd906e7862bb5f41ff9a', 'specimen id': 'SAN00001954', 'collected by': 'Andrew J. Mongue', 'insdc status': 'public', 'project name': 'DTOL', 'relationship': 'Offspring of wild-collected line FA', 'submitter id': '61b9bd906e7862bb5f41ff9a', 'gal_sample_id': 'SAN00001954', 'identified by': 'Andrew J. Mongue', 'organism part': 'WHOLE ORGANISM', 'sample same as': 'SAMEA12110511', 'collection date': '2021-10-21', 'ena last update': '2022-01-04', 'barcoding center': 'NOT COLLECTED', 'ena first public': '2022-01-04', 'specimen voucher': 'NOT COLLECTED', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2022-01-04T15:41:37Z', 'insdc center alias': 'EarlhamInstitute', 'insdc first public': '2022-01-04T15:41:37Z', 'collecting institution': 'University of Edinburgh', 'identifier_affiliation': 'University of Edinbrgh', 'original collection date': '2021-10-21', 'original collection location': 'United Kingdom | Scotland | Edinburgh | University of Edinburgh | Ashworth laboratories', 'geographic location (latitude)': '55.923954', 'geographic location (longitude)': '-3.172453', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'Scotland | Edinburgh | University of Edinburgh | Ashworth laboratories'}