Phragmites australis

RNA-seq of stem tissue from native Phragmites australis under T50 condition (R3A) (desert ecotype)

Total RNA was extracted using the NucleoSpin Plant RNA Isolation Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer and NanoDrop. Libraries were prepared by mRNA enrichment, followed by RNA fragmentation and reverse transcription to double-stranded cDNA using N6 random primers. After end repair and A-tailing, adaptors were ligated and the products were PCR-amplified. The single-stranded DNA was cyclized using splint oligos and sequenced the BGISEQ platform with PE100 reads.

{'age': 'Approximately 6 weeks post-germination', 'tissue': 'Stem', 'ecotype': 'Abu Dhabi, United Arab Emirates (desert ecotype)', 'isolate': 'Individual seedling from native P. australis population (Khalifa B, Abu Dhabi, UAE) (T50 treatment replicate R3A)', 'cultivar': 'Wild type', 'dev_stage': 'Seedling (0.5 meters tall)', 'treatment': 'T50 (50 mg/L imidacloprid)', 'sample_type': 'tissue', 'collected_by': 'Zayed University, Department of Environmental Sciences and Sustainability (ESS)', 'geo_loc_name': 'United Arab Emirates:Abu Dhabi', 'biosamplemodel': 'Plant', 'collection_date': '2023-04-30', 'growth_protocol': 'Seeds of native Phragmites australis collected from a natural population in the Khalifa B area of Abu Dhabi, UAE, were germinated in potting soil under greenhouse conditions. The seeds were soaked in water and covered with perforated polythene to maintain moisture for 2 weeks. Seedlings were then transferred to individual pots and grown in trays with watering three times per week. Plants were grown until they reached approximately 0.5 meters in height, then treated with imidacloprid at concentrations of 50 mg/L with constant irrigation for one week in trays, representing the T50 condition.', 'height_or_length': '0.5 meter', 'isolation_source': 'Stem from a seedling grown under greenhouse conditions from seeds of native Phragmites australis collected in Abu Dhabi, UAE', 'biomaterial_provider': 'Prof. Dr. Muhammad Naseem'}

RNA-seq of stem tissue from native Phragmites australis under T100 condition (R2A) (desert ecotype)

Total RNA was extracted using the NucleoSpin Plant RNA Isolation Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer and NanoDrop. Libraries were prepared by mRNA enrichment, followed by RNA fragmentation and reverse transcription to double-stranded cDNA using N6 random primers. After end repair and A-tailing, adaptors were ligated and the products were PCR-amplified. The single-stranded DNA was cyclized using splint oligos and sequenced the BGISEQ platform with PE100 reads.

{'age': 'Approximately 6 weeks post-germination', 'tissue': 'Stem', 'ecotype': 'Abu Dhabi, United Arab Emirates (desert ecotype)', 'isolate': 'Individual seedling from native P. australis population (Khalifa B, Abu Dhabi, UAE) (T100 treatment replicate R2A)', 'cultivar': 'Wild type', 'dev_stage': 'Seedling (0.5 meters tall)', 'treatment': 'T100 (100 mg/L imidacloprid)', 'sample_type': 'tissue', 'collected_by': 'Zayed University, Department of Environmental Sciences and Sustainability (ESS)', 'geo_loc_name': 'United Arab Emirates:Abu Dhabi', 'biosamplemodel': 'Plant', 'collection_date': '2023-04-30', 'growth_protocol': 'Seeds of native Phragmites australis collected from a natural population in the Khalifa B area of Abu Dhabi, UAE, were germinated in potting soil under greenhouse conditions. The seeds were soaked in water and covered with perforated polythene to maintain moisture for 2 weeks. Seedlings were then transferred to individual pots and grown in trays with watering three times per week. Plants were grown until they reached approximately 0.5 meters in height, then treated with imidacloprid at concentrations of 100 mg/L with constant irrigation for one week in trays, representing the T100 condition.', 'height_or_length': '0.5 meter', 'isolation_source': 'Stem from a seedling grown under greenhouse conditions from seeds of native Phragmites australis collected in Abu Dhabi, UAE', 'biomaterial_provider': 'Prof. Dr. Muhammad Naseem'}

RNA-seq of stem tissue from native Phragmites australis under T100 condition (R3A) (desert ecotype)

Total RNA was extracted using the NucleoSpin Plant RNA Isolation Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer and NanoDrop. Libraries were prepared by mRNA enrichment, followed by RNA fragmentation and reverse transcription to double-stranded cDNA using N6 random primers. After end repair and A-tailing, adaptors were ligated and the products were PCR-amplified. The single-stranded DNA was cyclized using splint oligos and sequenced the BGISEQ platform with PE100 reads.

{'age': 'Approximately 6 weeks post-germination', 'tissue': 'Stem', 'ecotype': 'Abu Dhabi, United Arab Emirates (desert ecotype)', 'isolate': 'Individual seedling from native P. australis population (Khalifa B, Abu Dhabi, UAE) (T100 treatment replicate R3A)', 'cultivar': 'Wild type', 'dev_stage': 'Seedling (0.5 meters tall)', 'treatment': 'T100 (100 mg/L imidacloprid)', 'sample_type': 'tissue', 'collected_by': 'Zayed University, Department of Environmental Sciences and Sustainability (ESS)', 'geo_loc_name': 'United Arab Emirates:Abu Dhabi', 'biosamplemodel': 'Plant', 'collection_date': '2023-04-30', 'growth_protocol': 'Seeds of native Phragmites australis collected from a natural population in the Khalifa B area of Abu Dhabi, UAE, were germinated in potting soil under greenhouse conditions. The seeds were soaked in water and covered with perforated polythene to maintain moisture for 2 weeks. Seedlings were then transferred to individual pots and grown in trays with watering three times per week. Plants were grown until they reached approximately 0.5 meters in height, then treated with imidacloprid at concentrations of 100 mg/L with constant irrigation for one week in trays, representing the T100 condition.', 'height_or_length': '0.5 meter', 'isolation_source': 'Stem from a seedling grown under greenhouse conditions from seeds of native Phragmites australis collected in Abu Dhabi, UAE', 'biomaterial_provider': 'Prof. Dr. Muhammad Naseem'}