Xanthomonas citri pv. glycines

RNA Seq of Xag wt in PCD inducing medium

Total RNA extraction was performed using TriReagent (Sigma, USA), as per the manufacturers protocol. The RNA quality was assessed using formaldehyde 1% agarose gel, and RNA integrity number. The library was prepared and Illumina-HiSeq2500 Rapid mode was utilised for sequencing by synthesis, generating around 20 million paired end reads, with minimum 150 bp read length per sample. At least 90% of reads in all the samples had QC value>30.

{'host': 'Soybean plant', 'strain': 'wild type', 'isolate': 'grown in PCD inducing medium', 'sample_type': 'Bacterial cells', 'geo_loc_name': 'India: Trombay', 'biosamplemodel': 'Microbe, viral or environmental', 'isolation_source': 'culture'}

RNA Seq of Xag AM2 WT treated with putative QSM

Total RNA extraction was performed using TriReagent (Sigma, USA), as per the manufacturers protocol. The RNA quality was assessed using formaldehyde 1% agarose gel, and RNA integrity number. The library was prepared and Illumina-HiSeq2500 Rapid mode was utilised for sequencing by synthesis, generating around 20 million paired end reads, with minimum 150 bp read length per sample. At least 90% of reads in all the samples had QC value>30.

{'host': 'Soybean plant', 'strain': 'wild type', 'isolate': 'AM2', 'sample_type': 'RNA', 'geo_loc_name': 'India: Maharashtra', 'biosamplemodel': 'Microbe, viral or environmental', 'collection_date': '2021', 'isolation_source': 'culture S4A', 'biomaterial_provider': 'Nilantana C. Bandyopadhyay'}

RNA Seq of Xag AM2 WT treated with putative QSM

Total RNA extraction was performed using TriReagent (Sigma, USA), as per the manufacturers protocol. The RNA quality was assessed using formaldehyde 1% agarose gel, and RNA integrity number. The library was prepared and Illumina-HiSeq2500 Rapid mode was utilised for sequencing by synthesis, generating around 20 million paired end reads, with minimum 150 bp read length per sample. At least 90% of reads in all the samples had QC value>30.

{'host': 'Soybean plant', 'strain': 'wild type', 'isolate': 'AM2', 'sample_type': 'RNA', 'geo_loc_name': 'India: Maharashtra', 'biosamplemodel': 'Microbe, viral or environmental', 'collection_date': '2021', 'isolation_source': 'culture S4B', 'biomaterial_provider': 'Nilantana C. Bandyopadhyay'}