Amrasca biguttula

De Novo transcriptome of Amrasca biguttula

The RNA Se paired end sequencing library were prepared from QC passed RNA using illumina TruSeq stranded mRNA sample prepration Kit. The 1st strand cDNA was synthesized to second using second strand mix and Act-D mix to facilitate RNA dependent syntheisi.. The PCR enriched libraries were analyzed in 4200 tape station system. follwowed by cluster generation and sequencing onto NextSeq500. The sequences were processed and subjected to routine bioinformatics procudures such as filtration of raw reads, Hq reads, de novo transcriptome assembly, transcript validation, CDS prediction. The functional annotation was done using BlastX, gene ontology using Blast2Go and pathway analysisi using KAAS.

{'sex': 'pooled male and female', 'host': 'Cotton', 'tissue': 'Whole body tisue', 'isolate': 'Punjab-Faridkot', 'lat_lon': '30.5932 N 74.8273 E', 'dev_stage': 'nymphs', 'env_biome': 'Cultivated cotton land', 'sub specie': 'biguttula', 'sub_species': 'biguttula', 'collected_by': 'Satnam Singh', 'geo_loc_name': 'India: Punjab, Faridkot PAU Regional station Cotton field', 'identified_by': 'Satnam singh', 'biosamplemodel': 'Invertebrate', 'collection_date': '2016-07-01', 'host_tissue_sampled': 'Cotton plants', 'biomaterial_provider': 'Satnam Singh'}