Botrytis cinerea

Botrytis-cinerea treated with osthole for 36 hours, with a final concentration of EC50

Mycelial plugs (4 mm diameter) from the edge of 3-day-old colony of the CGMCC3.20932 was transferred to PDB medium culture for two days,with treated with DMSO , and inoculated in a shake cultivationat 25C for 12h. The RNA of the total sample was isolated and purified by TRIzol (thermofisher, 15596018) according to the operation scheme provided by the manufacturer. Then the quantity and purity of total RNA was controlled by NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) and the integrity of RNA was detected by Bioanalyzer 2100 (Agilent, CA, USA). The concentration was >50ng/L, RIN value >7.0, and total RNA>9g.

{'strain': 'CGMCC3.20932', 'isolate': 'Botrytis cinerea mycelium sample 6', 'lat_lon': '28.11 N 107.30 E', 'collected_by': 'Zhuo Chen', 'geo_loc_name': 'China: Wuchuan County, Guizhou Province', 'biosamplemodel': 'Pathogen.env', 'collection_date': '2025-04', 'isolation_source': 'Tomato leaves and fruit'}

RNA-seq of Botrytis cinerea: mycelium

Mix total RNA

{'strain': 'KY-1, KY-1V1, B05.10', 'sample_type': 'cell culture', 'biosamplemodel': 'Microbe, viral or environmental'}

transcriptsome of wild type Botrytis cinerea strain B05.10

extract RNA with tiangen extract kit

{'strain': 'B05.10', 'lat_lon': '30.25 N 119.65 E', 'collected_by': 'Dan Wang', 'geo_loc_name': 'China:Zhejiang', 'biosamplemodel': 'Pathogen.env', 'collection_date': '2024-11-20', 'isolation_source': 'campus of ZAFU'}