Cyperus esculentus

Leaves with PEG treated for 2nd time

Leaves with PEG treated for 2nd time

{'age': 'seeding stage', 'tissue': 'Leaves', 'ecotype': 'Jisha 2', 'isolate': 'Leaves with PEG treated for 2nd time', 'variety': 'sativus', 'cultivar': 'Tiger nut', 'dev_stage': 'seeding stage', 'geo_loc_name': 'China: Jilin', 'biosamplemodel': 'Plant', 'collection_date': '2023-10'}

Leaves with PEG treated for 3rd time

Leaves with PEG treated for 3rd time

{'age': 'seeding stage', 'tissue': 'Leaves', 'ecotype': 'Jisha 2', 'isolate': 'Leaves with PEG treated for 3rd time', 'variety': 'sativus', 'cultivar': 'Tiger nut', 'dev_stage': 'seeding stage', 'geo_loc_name': 'China: Jilin', 'biosamplemodel': 'Plant', 'collection_date': '2023-10'}

Roots with PEG treated for 1st time

Roots with PEG treated for 1st time

{'age': 'seeding stage', 'tissue': 'Roots', 'ecotype': 'Jisha 2', 'isolate': 'Roots with PEG treated for 1st time', 'variety': 'sativus', 'cultivar': 'Tiger nut', 'dev_stage': 'seeding stage', 'geo_loc_name': 'China: Jilin', 'biosamplemodel': 'Plant', 'collection_date': '2023-10'}

YC164-2-3

Full-length cDNA was synthesize by NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module. Full-length cDNA molecules were then amplified by PCR. Harvested cDNA were processed for end-repairing/A-Tailing. SMRT hairpin adapters were ligated to the end of double-stranded cDNA molecules.

{'tissue': 'tuber', 'isolate': 'YC164-2-3', 'dev_stage': 'not provided', 'geo_loc_name': 'China:Jilin Province', 'biosamplemodel': 'Plant', 'collection_date': '2022', 'isolation_source': 'plant', 'biomaterial_provider': 'Jilin Academy of Agricultural Sciences'}

CK-2

mRNA was isolated by Oligo(dT)-attached magnetic beads. mRNA was then randomly fragmented in fragmentation buffer. First-strand cDNA was synthesized with fragmented mRNA as template and random hexamers as primers, followed by second-strand synthesis with addition of PCR buffer, dNTPs, RNase H and DNA polymerase I. Purification of cDNA was processed with AMPure XP beads. Double-strand cDNA was subjected to end repair. Adenosine was added to the end and ligated to adapters. AMPure XP beads were applied here to select fragments within certain size range. cDNA library was obtained by certain rounds of PCR on cDNA fragments.

{'tissue': 'tuber', 'isolate': 'CK-2', 'dev_stage': 'not provided', 'geo_loc_name': 'China:Jilin Province', 'biosamplemodel': 'Plant', 'collection_date': '2022', 'isolation_source': 'plant', 'biomaterial_provider': 'Jilin Academy of Agricultural Sciences'}

YC164-2

mRNA was isolated by Oligo(dT)-attached magnetic beads. mRNA was then randomly fragmented in fragmentation buffer. First-strand cDNA was synthesized with fragmented mRNA as template and random hexamers as primers, followed by second-strand synthesis with addition of PCR buffer, dNTPs, RNase H and DNA polymerase I. Purification of cDNA was processed with AMPure XP beads. Double-strand cDNA was subjected to end repair. Adenosine was added to the end and ligated to adapters. AMPure XP beads were applied here to select fragments within certain size range. cDNA library was obtained by certain rounds of PCR on cDNA fragments.

{'tissue': 'tuber', 'isolate': 'YC164-2', 'dev_stage': 'not provided', 'geo_loc_name': 'China:Jilin Province', 'biosamplemodel': 'Plant', 'collection_date': '2022', 'isolation_source': 'plant', 'biomaterial_provider': 'Jilin Academy of Agricultural Sciences'}