Phthorimaea operculella

RNA-seq of male adult reproductive organs 1

The total RNA of the tissues above was separately extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturers instructions. RNA integrity was determined with an Agilent Bioanalyzer 2100 system (Agilent Technologies Inc., CA, USA). RNA concentration and purity were measured by a Nanodrop ND2000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE). One microgram of total RNA per sample was used for cDNA library construction. A cDNA library was prepared using the Optimal Dual-mode mRNA Library Prep Kit (BGI, Shenzhen, China) according to the manufacturer's instructions. The library was then sequenced on the BGI MGISEQ-2000 platform (BGI, Shenzhen, China), and paired-end (150bp) reads were generated.

{'host': 'potato tuber', 'tissue': 'male adult reproductive organs 1', 'isolate': 'male adult reproductive organs 1', 'geo_loc_name': 'China: BeiJing', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-02', 'isolation_source': 'Labtatory populations'}

RNA-seq of male adult reproductive organs 2

The total RNA of the tissues above was separately extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturers instructions. RNA integrity was determined with an Agilent Bioanalyzer 2100 system (Agilent Technologies Inc., CA, USA). RNA concentration and purity were measured by a Nanodrop ND2000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE). One microgram of total RNA per sample was used for cDNA library construction. A cDNA library was prepared using the Optimal Dual-mode mRNA Library Prep Kit (BGI, Shenzhen, China) according to the manufacturer's instructions. The library was then sequenced on the BGI MGISEQ-2000 platform (BGI, Shenzhen, China), and paired-end (150bp) reads were generated.

{'host': 'potato tuber', 'tissue': 'male adult reproductive organs 2', 'isolate': 'male adult reproductive organs 2', 'geo_loc_name': 'China: BeiJing', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-02', 'isolation_source': 'Labtatory populations'}

RNA-seq of male adult reproductive organs 3

The total RNA of the tissues above was separately extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturers instructions. RNA integrity was determined with an Agilent Bioanalyzer 2100 system (Agilent Technologies Inc., CA, USA). RNA concentration and purity were measured by a Nanodrop ND2000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE). One microgram of total RNA per sample was used for cDNA library construction. A cDNA library was prepared using the Optimal Dual-mode mRNA Library Prep Kit (BGI, Shenzhen, China) according to the manufacturer's instructions. The library was then sequenced on the BGI MGISEQ-2000 platform (BGI, Shenzhen, China), and paired-end (150bp) reads were generated.

{'host': 'potato tuber', 'tissue': 'male adult reproductive organs 3', 'isolate': 'male adult reproductive organs 3', 'geo_loc_name': 'China: BeiJing', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-02', 'isolation_source': 'Labtatory populations'}