Leucoptera coffeella

Hapsifera sp. transcriptome

Nucleic acids were extracted using Promega SV total RNA isolation mini-kits either with or without DNase digestion. Following DNase digestion, the RNA-only preps were subjected to poly-A selection and indexed library construction. The remaining extracts of total nucleic acids were used to produce cDNAs with low-input Clontech kits for either poly-dT priming (SMARTer Ultra Low input RNA kit Đv3, #634849) or universal priming (#634940). Following shearing to 200-bp size with a Covaris instrument, cDNA fragments were used for indexed library construction (Clontech kit #634947 for low input). Libraries were left unnormalized so as to favor highly expressed genes likely to be present in most species and all life stages.

{'tissue': 'whole specimen', 'isolate': 'CWM-07-2322', 'dev_stage': 'adult', 'geo_loc_name': 'Colombia', 'biosamplemodel': 'Invertebrate', 'collection_date': '2007-10-15', 'isolation_source': 'lab culture', 'specimen_voucher': 'CWM-07-2322'}

RNAseq of coffee leafminer (adult male)

pooled sample of whole insects

{'host': 'Coffea arabica', 'tissue': 'whole insects', 'isolate': 'Planaltina', 'dev_stage': 'Adult_M', 'geo_loc_name': 'Brazil', 'biosamplemodel': 'Invertebrate', 'collection_date': '2022', 'isolation_source': 'Embrapa Cerrados Planaltina'}

RNAseq of coffee leafminer (adult female)

pooled sample of whole insects

{'host': 'Coffea arabica', 'tissue': 'whole insects', 'isolate': 'Planaltina', 'dev_stage': 'Adult_F', 'geo_loc_name': 'Brazil', 'biosamplemodel': 'Invertebrate', 'collection_date': '2022', 'isolation_source': 'Embrapa Cerrados Planaltina'}