Uromyces transversalis

Uromyces transversalis transcriptiome of germinating urediniospores

RNA was extracted from urediniospores isolated from infected leaves of Gladiolus communis. PolyA-enriched total RNA was used to prepare a library optimized for Roche/454 Titanium sequencing that included partial normalization with double-stranded nuclease and PCR suppression of misligated products. Reads were cleaned of adaptor sequences using http://sourceforge.net/projects/estclean. See doi: 10.1111/pbi.12068

{'host': 'Gladiolus communis', 'isolate': 'CA-07', 'lat_lon': '33.257476 N 117.198222 W', 'lab_host': 'Gladiolus communis', 'sample_size': 'several leaves', 'sample_type': 'urediniospores', 'collected_by': 'Pat Nolan, Dept. of Agriculture, Countv of San Diego CA USA', 'geo_loc_name': 'USA: Vista, CA', 'identified_by': 'Pat Nolan, Dept. of Agriculture, Countv of San Diego CA, USA', 'biosamplemodel': 'Microbe, viral or environmental', 'collection_date': '2007-06-15', 'passage_history': 'TNTC; urediniospores collected from leaf surface by microvaccuuming, and inoculation of fresh host on a biweekly schedule since material received', 'isolation_source': 'Commercial Nursery, Gopher Canyon Rd., Vista, CA', 'host_tissue_sampled': 'infected Gladiolus communis leaf material with erumpent pustules containing urediniospores', 'biomaterial_provider': 'Douglas Luster, USDA ARS - Ft. Detrick, MD'}