Busseola fusca

RNA sequencing of Busseola fusca 4th instar larva

RNA extractions were performed on 10 different B. fusca tissues types, (thoraces, antennae [both sexes], and female ovipositor), developmental stages (eggs, neonates, fourth instar larvae, and adults [both sexes]), and parasitoid-exposure conditions (unexposed and exposed to two strains of wasp). All samples were put in RNAlater stabilizing solution (Invitrogen cat. No. AM 7020) and kept at -80C until RNA extraction. During RNA extraction, each sample was quickly ground into a fine powder with liquid nitrogen, and total RNA was prepared using TRIzol Reagent (Invitrogen cat No 15596-026) with slight modifications. RNA quantification was performed by bioanalyzer RNA kits (Agilent Technologies) and quality was further confirmed by gel electrophoresis. We used a TruSeq RNA Library Prep Kit (Illumina) to prepare libraries for each sample, with an enrichment step to target mRNA. We performed next-generation sequencing using the Illumina MiSeq platform at the Biosciences eastern and central Africa (BecA) genomics platform at the International Livestock Research Institute (ILRI) in Nairobi, Kenya.

{'tissue': 'whole body', 'dev_stage': '4th instar larva', 'geo_loc_name': 'Kenya', 'biosamplemodel': 'Invertebrate', 'collection_date': '2008', 'isolation_source': 'maize', 'parasitoid_exposure': 'Unexposed'}

RNA sequencing of Busseola fusca 4th instar larva exposed to C. sesamiae Kitale (Cs-inland)

RNA extractions were performed on 10 different B. fusca tissues types, (thoraces, antennae [both sexes], and female ovipositor), developmental stages (eggs, neonates, fourth instar larvae, and adults [both sexes]), and parasitoid-exposure conditions (unexposed and exposed to two strains of wasp). All samples were put in RNAlater stabilizing solution (Invitrogen cat. No. AM 7020) and kept at -80C until RNA extraction. During RNA extraction, each sample was quickly ground into a fine powder with liquid nitrogen, and total RNA was prepared using TRIzol Reagent (Invitrogen cat No 15596-026) with slight modifications. RNA quantification was performed by bioanalyzer RNA kits (Agilent Technologies) and quality was further confirmed by gel electrophoresis. We used a TruSeq RNA Library Prep Kit (Illumina) to prepare libraries for each sample, with an enrichment step to target mRNA. We performed next-generation sequencing using the Illumina MiSeq platform at the Biosciences eastern and central Africa (BecA) genomics platform at the International Livestock Research Institute (ILRI) in Nairobi, Kenya.

{'tissue': 'whole body', 'dev_stage': '4th instar larva', 'geo_loc_name': 'Kenya', 'biosamplemodel': 'Invertebrate', 'collection_date': '2008', 'isolation_source': 'maize', 'parasitoid_exposure': 'Exposed to C. sesamiae Kitale (Cs-inland)'}

RNA sequencing of Busseola fusca 4th instar larva exposed to C. sesamiae Mombasa (Cs-coast)

RNA extractions were performed on 10 different B. fusca tissues types, (thoraces, antennae [both sexes], and female ovipositor), developmental stages (eggs, neonates, fourth instar larvae, and adults [both sexes]), and parasitoid-exposure conditions (unexposed and exposed to two strains of wasp). All samples were put in RNAlater stabilizing solution (Invitrogen cat. No. AM 7020) and kept at -80C until RNA extraction. During RNA extraction, each sample was quickly ground into a fine powder with liquid nitrogen, and total RNA was prepared using TRIzol Reagent (Invitrogen cat No 15596-026) with slight modifications. RNA quantification was performed by bioanalyzer RNA kits (Agilent Technologies) and quality was further confirmed by gel electrophoresis. We used a TruSeq RNA Library Prep Kit (Illumina) to prepare libraries for each sample, with an enrichment step to target mRNA. We performed next-generation sequencing using the Illumina MiSeq platform at the Biosciences eastern and central Africa (BecA) genomics platform at the International Livestock Research Institute (ILRI) in Nairobi, Kenya.

{'tissue': 'whole body', 'dev_stage': '4th instar larva', 'geo_loc_name': 'Kenya', 'biosamplemodel': 'Invertebrate', 'collection_date': '2008', 'isolation_source': 'maize', 'parasitoid_exposure': 'Exposed to C. sesamiae Mombasa (Cs-coast)'}