Callosobruchus chinensis

RNA-seq of callosobruchus chinensis :adult female and male

Transcriptome sequencing of the callosobruchus chinensis,replication 2 of cold stress

{'age': '2 days of age', 'sex': 'pooled male and female', 'tissue': 'whole organism', 'ecotype': 'shanxi', 'isolate': 'Insect', 'sample_type': 'whole organism', 'geo_loc_name': 'China:Jinzhong', 'biosamplemodel': 'Model organism or animal'}

Control

Method: The insects in the control group were exposed to acetone as a control, and on the 7th day, they were sacrificed for the sequencing process. sample was sequenced on the Illumina platform using 2 x 150 bp chemistry. data The sequenced raw data were processed to obtain high-quality concordant reads using Trimmomatic v0.39 and an in-house script to remove adapters, ambiguous reads (reads with unknown nucleotides N larger than 5%), and low-quality sequences (reads with more than 10% quality threshold (QV) < 25 phred score). The resulting high quality (QV>25), paired 10% quality threshold (QV) < 25 phred score). The resulting high-quality (QV > 25) paired-end reads were used for de novo assembly of the samples.

{'tissue': 'Whole Body', 'dev_stage': 'adult', 'geo_loc_name': 'India: Gujarat', 'biosamplemodel': 'Invertebrate'}

Deltamethrin Treated

Method: The insects in the treated group were exposed to deltamethrin, and on the 7th day, they were sacrificed for the sequencing process. sample was sequenced on the Illumina platform using 2 x 150 bp chemistry. data The sequenced raw data were processed to obtain high-quality concordant reads using Trimmomatic v0.39 and an in-house script to remove adapters, ambiguous reads (reads with unknown nucleotides N larger than 5%), and low-quality sequences (reads with more than 10% quality threshold (QV) < 25 phred score). The resulting high quality (QV>25), paired 10% quality threshold (QV) < 25 phred score). The resulting high-quality (QV > 25) paired-end reads were used for de novo assembly of the samples.

{'tissue': 'Whole Body', 'geo_loc_name': 'India: Gujarat', 'biosamplemodel': 'Invertebrate'}