Puccinia horiana

Puccinia horiana transcriptome of germinating basidiospores

RNA was extracted from teliospores isolated from infected leaves of Chrysanthemum X morifolium. PolyA-enriched total RNA was used to prepare a library optimized for Roche/454 Titanium sequencing that included partial normalization with double-stranded nuclease and PCR suppression of misligated products. Reads were cleaned of adaptor sequences using http://sourceforge.net/projects/estclean. See doi: 10.1111/pbi.12068

{'host': 'Chrysanthemum x morifolium', 'isolate': 'PA-11', 'lat_lon': '41.289133 N 75.833899 W', 'lab_host': 'Chrysanthemum X morifolium', 'samp_size': 'several leaves', 'sample_type': 'germinating basidiospores', 'collected_by': 'Seong-Hwan Kim, PA Department of Agriculture', 'geo_loc_name': 'USA: Wilkes Barre, PA', 'identified_by': 'Seong-Hwan Kim', 'biosamplemodel': 'Microbe, viral or environmental', 'collection_date': '2011-09-28', 'passage_history': 'multiple; basidiospores collected from leaf surface by microvaccuuming, and inoculation of fresh host on a biweekly schedule since material received', 'isolation_source': 'Greenhouse, Wilkes Barre, PA, USA', 'host_tissue_sampled': 'infected Chrysanthemum X morifolium leaf material with erumpent pustules containing teliospores', 'biomaterial_provider': 'Douglas Luster, USDA ARS - Ft. Detrick, MD'}