Heterotermes tenuis

RNA-Seq from workers of Heterotermes tenuis

Brazilian termites of three species, Heterotermes tenuis (Ht), Cornitermes cumulans (Cc) and Velocitermes heteropterus (Vh), were collected in the Southeast region of Brazil (2223S, 4731W - Ht and Cc and 2142S, 4728W - Vh). Workers and soldiers of the same species were sampled in the same colony and cDNA libraries were constructed separately for each caste. For this, the whole body of 200 individuals was macerated with liquid nitrogen and total RNA was extracted and purified with RNeasy Mini Kit (QIAGEN - Cat. n 74106), according to manufacturer's instructions. RNA-seq libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina) protocol, according to manufacturer's guidelines. Libraries of 2 x 100 bp paired-end reads were sequenced on Illumina HiSeqTM2000 at the Laboratory of Functional Genomics Applied to Agriculture and Agrienergy, ESALQ-USP, Brazil.

{'tissue': 'Whole body', 'isolate': 'Worker', 'geo_loc_name': 'Brazil: Sao Paulo', 'biosamplemodel': 'Invertebrate', 'collection_date': '2011', 'isolation_source': 'Colony from field'}

RNA-Seq from soldiers of Heterotermes tenuis

Brazilian termites of three species, Heterotermes tenuis (Ht), Cornitermes cumulans (Cc) and Velocitermes heteropterus (Vh), were collected in the Southeast region of Brazil (2223S, 4731W - Ht and Cc and 2142S, 4728W - Vh). Workers and soldiers of the same species were sampled in the same colony and cDNA libraries were constructed separately for each caste. For this, the whole body of 200 individuals was macerated with liquid nitrogen and total RNA was extracted and purified with RNeasy Mini Kit (QIAGEN - Cat. n 74106), according to manufacturer's instructions. RNA-seq libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina) protocol, according to manufacturer's guidelines. Libraries of 2 x 100 bp paired-end reads were sequenced on Illumina HiSeqTM2000 at the Laboratory of Functional Genomics Applied to Agriculture and Agrienergy, ESALQ-USP, Brazil.

{'tissue': 'Whole body', 'isolate': 'Soldier', 'geo_loc_name': 'Brazil: Sao Paulo', 'biosamplemodel': 'Invertebrate', 'collection_date': '2011', 'isolation_source': 'Colony from field'}