Bactrocera correcta

RNA-seq of Bactrocera correcta at 25 degree celsius replicate 3

We extracted RNA from three groups of thirty larvae after exposing to specific temperatures (25C, 35C and 38C) for 4h followed by 25 C for 1 h. The quantity and integrity of the RNA were assessed with a Qubit RNA Assay Kit in Qubit2.0 Flurometer (Life Technologies, CA, USA) and an RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). All libraries ware prepared using NEBNextUltra RNA Library Prep Kit for Illumina (NEB, USA) according to the manufacturers protocol and were sequenced at the Biomarker Technologies company (Beijing, China). The clustering of index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) and paired-end reads were generated through sequencing and the library preparations on an Illumina Hiseq 2000 platform after cluster generation.

{'age': '3rd instar larvae', 'host': 'Citrus sinensis', 'tissue': 'Whole body', 'isolate': 'Yunnan', 'collected_by': 'Xinyue9', 'geo_loc_name': 'China: Yunnan', 'biosamplemodel': 'Invertebrate', 'collection_date': '2014-10-01'}

adult female antennae

mRNA isolation and cDNA library preparation were carried out using the Illumina mRNA sequencing kit.

{'tissue': 'antennae', 'isolate': 'Laboratory strain', 'geo_loc_name': 'China: Honghe', 'biosamplemodel': 'Invertebrate', 'collection_date': '2014-09', 'isolation_source': 'Guangzhou'}

adult male antennae

mRNA isolation and cDNA library preparation were carried out using the Illumina mRNA sequencing kit.

{'tissue': 'antennae', 'isolate': 'Laboratory strain', 'geo_loc_name': 'China: Honghe', 'biosamplemodel': 'Invertebrate', 'collection_date': '2014-09', 'isolation_source': 'Guangzhou'}