Sagittaria sagittifolia

RNA-Seq of Sagittaria sagittifolia: flower bud

After the sample test is qualified, the main process is as follows:(1) Eukaryotic mRNA; is enriched with magnetic beads with Oligo (dT)(2) Add the Fragmentation Buffer to randomly interrupt the mRNA;(3) The first cDNA chain is synthesized with the six-base random primer (random hexamers), then synthesized by buffer, dNTPs, RNase H and DNA polymerase I, and the cDNA; is purified by AMPure XP beads(4) Purified double-chain cDNA performs end repair, add A tail and connect the sequencing connector, and then make segment size selection with AMPure XP beads;(5) Finally, the cDNA library is obtained through PCR enrichment.1.2.3 text library quality controlAfter the completion of the library construction, the quality of the library is tested with advanced equipment in molecular biology, and the computer sequencing can be conducted after the test results meet the requirements.1.2.4 on-computer sequencingAfter passing the library inspection, different libraries are sequenced by pooling, with Illumina platform according to the target offline data volume

{'tissue': 'flower buds', 'cultivar': 'Flore Pleno', 'dev_stage': 'flower bud', 'geo_loc_name': 'China: the Guangxi Academy of Agricultural Sciences, Guangxi Zhuangzuzihiqu', 'biosamplemodel': 'Plant'}

RNA-Seq of Sagittaria sagittifolia: flower bud

After the sample test is qualified, the main process is as follows:(1) Eukaryotic mRNA; is enriched with magnetic beads with Oligo (dT)(2) Add the Fragmentation Buffer to randomly interrupt the mRNA;(3) The first cDNA chain is synthesized with the six-base random primer (random hexamers), then synthesized by buffer, dNTPs, RNase H and DNA polymerase I, and the cDNA; is purified by AMPure XP beads(4) Purified double-chain cDNA performs end repair, add A tail and connect the sequencing connector, and then make segment size selection with AMPure XP beads;(5) Finally, the cDNA library is obtained through PCR enrichment.1.2.3 text library quality controlAfter the completion of the library construction, the quality of the library is tested with advanced equipment in molecular biology, and the computer sequencing can be conducted after the test results meet the requirements.1.2.4 on-computer sequencingAfter passing the library inspection, different libraries are sequenced by pooling, with Illumina platform according to the target offline data volume

{'tissue': 'flower buds', 'cultivar': 'Flore Pleno', 'dev_stage': 'flower bud', 'geo_loc_name': 'China: the Guangxi Academy of Agricultural Sciences, Guangxi Zhuangzuzihiqu', 'biosamplemodel': 'Plant'}

RNA-Seq of Sagittaria sagittifolia: flower bud

After the sample test is qualified, the main process is as follows:(1) Eukaryotic mRNA; is enriched with magnetic beads with Oligo (dT)(2) Add the Fragmentation Buffer to randomly interrupt the mRNA;(3) The first cDNA chain is synthesized with the six-base random primer (random hexamers), then synthesized by buffer, dNTPs, RNase H and DNA polymerase I, and the cDNA; is purified by AMPure XP beads(4) Purified double-chain cDNA performs end repair, add A tail and connect the sequencing connector, and then make segment size selection with AMPure XP beads;(5) Finally, the cDNA library is obtained through PCR enrichment.1.2.3 text library quality controlAfter the completion of the library construction, the quality of the library is tested with advanced equipment in molecular biology, and the computer sequencing can be conducted after the test results meet the requirements.1.2.4 on-computer sequencingAfter passing the library inspection, different libraries are sequenced by pooling, with Illumina platform according to the target offline data volume

{'tissue': 'flower buds', 'cultivar': 'Flore Pleno', 'dev_stage': 'flower bud', 'geo_loc_name': 'China: the Guangxi Academy of Agricultural Sciences, Guangxi Zhuangzuzihiqu', 'biosamplemodel': 'Plant'}