Peregrinus maidis

RNA-Seq of Peregrinus maidis: short-winged females

Total RNA was isolated from a range of P. maidis life stages: early-stage embryos (n = 180), mid- and late-stage embryos (n = 80 each), first- second- and third-stage nymphs (n = 20 each), fourth- and fifth-stage nymphs (n = 20 each), as well as short-winged adult males (n = 20), short-winged adult females (n = 20), long-winged adult males (n = 20), and long-winged adult females (n = 20) using the Qiagen RNeasy mini kit (Qiagen, Hilden, Germany) and treated with DNase I (Qiagen, Hilden, Germany) according to the manufacturers instructions. The quality of each total RNA sample was assessed using an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). Then, samples were submitted to the Genomic Sciences Laboratory (North Carolina State University, NC) for poly-A selection, fragmentation (~650 bp), and Illumina TruSeq library preparation. The resulting eight libraries were 300 bp paired-end sequenced on an Illumina MiSeq sequencer (Illumina, San Diego, CA).

{'age': 'short-winged females', 'tissue': 'whole body', 'geo_loc_name': 'USA: North Carolina', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018', 'isolation_source': 'PVVI Lab'}

RNA-Seq of Peregrinus maidis: long-winged males

Total RNA was isolated from a range of P. maidis life stages: early-stage embryos (n = 180), mid- and late-stage embryos (n = 80 each), first- second- and third-stage nymphs (n = 20 each), fourth- and fifth-stage nymphs (n = 20 each), as well as short-winged adult males (n = 20), short-winged adult females (n = 20), long-winged adult males (n = 20), and long-winged adult females (n = 20) using the Qiagen RNeasy mini kit (Qiagen, Hilden, Germany) and treated with DNase I (Qiagen, Hilden, Germany) according to the manufacturers instructions. The quality of each total RNA sample was assessed using an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). Then, samples were submitted to the Genomic Sciences Laboratory (North Carolina State University, NC) for poly-A selection, fragmentation (~650 bp), and Illumina TruSeq library preparation. The resulting eight libraries were 300 bp paired-end sequenced on an Illumina MiSeq sequencer (Illumina, San Diego, CA).

{'age': 'long-winged males', 'tissue': 'whole body', 'geo_loc_name': 'USA: North Carolina', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018', 'isolation_source': 'PVVI Lab'}

RNA-Seq of Peregrinus maidis: long-winged females

Total RNA was isolated from a range of P. maidis life stages: early-stage embryos (n = 180), mid- and late-stage embryos (n = 80 each), first- second- and third-stage nymphs (n = 20 each), fourth- and fifth-stage nymphs (n = 20 each), as well as short-winged adult males (n = 20), short-winged adult females (n = 20), long-winged adult males (n = 20), and long-winged adult females (n = 20) using the Qiagen RNeasy mini kit (Qiagen, Hilden, Germany) and treated with DNase I (Qiagen, Hilden, Germany) according to the manufacturers instructions. The quality of each total RNA sample was assessed using an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). Then, samples were submitted to the Genomic Sciences Laboratory (North Carolina State University, NC) for poly-A selection, fragmentation (~650 bp), and Illumina TruSeq library preparation. The resulting eight libraries were 300 bp paired-end sequenced on an Illumina MiSeq sequencer (Illumina, San Diego, CA).

{'age': 'long-winged females', 'tissue': 'whole body', 'geo_loc_name': 'USA: North Carolina', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018', 'isolation_source': 'PVVI Lab'}