Hibiscus trionum

RNAseq of Proximal region of Stage 2 petal of Hibiscus trionum, plant 3, forward reads

Tissue was collected from five individual H. trionum CUBG plants to generate five biological replicates: for each biological replicate, stage 2 petals were harvested and dissected to separate proximal, boundary and distal region. Tissue from these 15 samples were flash frozen in liquid nitrogen, ground to a fine powder, and total RNA extracted using the Spectrum Plant Total RNA kit (Sigma STRN250) with 40 mg of starting material. An on-column DNase I (Sigma DNASE70) treatment was performed between the first two column washes. RNA was eluted with 30 l nuclease-free H2O (Promega MC1191). RNA purity was analyzed using a NanoDrop (ThermoFisher Scientific) and RNA ScreenTape (Agilent 5067-5576, 5067-5577, 5067-5578) on the Agilent TapeStation 2200 system. Samples with minimum 1.75 g total RNA (>50 ng.l-1), and A260/280 value of 2.0 were shipped on dry ice to Azenta Life Sciences, Leipzig (formerly GeneWiz) for RNA-seq library preparation and sequencing. Library preparation was performed with polyA selection and sequencing using an Illumina HiSeq machine (2x 150bp configuration, ~350M raw paired-end reads per lane, at least 30M reads per sample).

{'age': '4 weeks', 'tissue': 'Petal', 'ecotype': 'CUBG', 'isolate': 'Proximal region of Stage 2 petal of Hibiscus trionum, plant 3, forward reads', 'dev_stage': 'Stage 2', 'phenotype': 'wild-type', 'collected_by': 'May Yeo, Edwige Moyroud', 'geo_loc_name': 'United Kingdom: Cambridge', 'biosamplemodel': 'Plant', 'collection_date': '2018-01'}

RNAseq of Proximal region of Stage 2 petal of Hibiscus trionum, plant 4, forward reads

Tissue was collected from five individual H. trionum CUBG plants to generate five biological replicates: for each biological replicate, stage 2 petals were harvested and dissected to separate proximal, boundary and distal region. Tissue from these 15 samples were flash frozen in liquid nitrogen, ground to a fine powder, and total RNA extracted using the Spectrum Plant Total RNA kit (Sigma STRN250) with 40 mg of starting material. An on-column DNase I (Sigma DNASE70) treatment was performed between the first two column washes. RNA was eluted with 30 l nuclease-free H2O (Promega MC1191). RNA purity was analyzed using a NanoDrop (ThermoFisher Scientific) and RNA ScreenTape (Agilent 5067-5576, 5067-5577, 5067-5578) on the Agilent TapeStation 2200 system. Samples with minimum 1.75 g total RNA (>50 ng.l-1), and A260/280 value of 2.0 were shipped on dry ice to Azenta Life Sciences, Leipzig (formerly GeneWiz) for RNA-seq library preparation and sequencing. Library preparation was performed with polyA selection and sequencing using an Illumina HiSeq machine (2x 150bp configuration, ~350M raw paired-end reads per lane, at least 30M reads per sample).

{'age': '4 weeks', 'tissue': 'Petal', 'ecotype': 'CUBG', 'isolate': 'Proximal region of Stage 2 petal of Hibiscus trionum, plant 4, forward reads', 'dev_stage': 'Stage 2', 'phenotype': 'wild-type', 'collected_by': 'May Yeo, Edwige Moyroud', 'geo_loc_name': 'United Kingdom: Cambridge', 'biosamplemodel': 'Plant', 'collection_date': '2018-01'}

RNAseq of Proximal region of Stage 2 petal of Hibiscus trionum, plant 5, forward reads

Tissue was collected from five individual H. trionum CUBG plants to generate five biological replicates: for each biological replicate, stage 2 petals were harvested and dissected to separate proximal, boundary and distal region. Tissue from these 15 samples were flash frozen in liquid nitrogen, ground to a fine powder, and total RNA extracted using the Spectrum Plant Total RNA kit (Sigma STRN250) with 40 mg of starting material. An on-column DNase I (Sigma DNASE70) treatment was performed between the first two column washes. RNA was eluted with 30 l nuclease-free H2O (Promega MC1191). RNA purity was analyzed using a NanoDrop (ThermoFisher Scientific) and RNA ScreenTape (Agilent 5067-5576, 5067-5577, 5067-5578) on the Agilent TapeStation 2200 system. Samples with minimum 1.75 g total RNA (>50 ng.l-1), and A260/280 value of 2.0 were shipped on dry ice to Azenta Life Sciences, Leipzig (formerly GeneWiz) for RNA-seq library preparation and sequencing. Library preparation was performed with polyA selection and sequencing using an Illumina HiSeq machine (2x 150bp configuration, ~350M raw paired-end reads per lane, at least 30M reads per sample).

{'age': '4 weeks', 'tissue': 'Petal', 'ecotype': 'CUBG', 'isolate': 'Proximal region of Stage 2 petal of Hibiscus trionum, plant 5, forward reads', 'dev_stage': 'Stage 2', 'phenotype': 'wild-type', 'collected_by': 'May Yeo, Edwige Moyroud', 'geo_loc_name': 'United Kingdom: Cambridge', 'biosamplemodel': 'Plant', 'collection_date': '2018-01'}