Crioceris asparagi

RNA-Seq of Crioceris asparagi: adult beetle

Total RNA was extracted using the innuPrep DNA/RNA Mini kit (Analytik Jena, Jena, Germany) following the manufacturer's instructions. Genomic DNA contamination was removed by DNAse treatment (TURBO DNase, Invitrogen, Carlsbad, CAL, USA) for 30 min at 37 C. Total RNA samples were further purified by using the RNeasy MinElute Clean up Kit (Qiagen, Hilden, Germany) following the manufacturers protocol, except for elution to 20 l volume. The integrity and quality of the RNA samples were determined using the RNA 6000 Nano LabChip kit on an Agilent 2100 Bioanalyzer (both Agilent Technologies, Santa Clara, CAL, USA) according to the manufacturers instructions. RNA-Seq was outsourced to the Max Planck Genome center (Cologne, Germany). There, poly(A)+ enriched RNA was fragmented to an average of 300-350 nucleotides and a TruSeq compatible, directional library was prepared for each sample. Libraries were tagged using dual indexing and were multiplexed on the same sequencing lane. Sequencing was carried out on a HiSeq3000 sequencing platform (Illumina, CA, USA) using paired-end (2 x 150 bp) reads.

{'tissue': 'whole insect', 'isolate': 'wild type individual', 'geo_loc_name': 'Germany', 'biosamplemodel': 'Invertebrate', 'collection_date': '2019-05-15', 'isolation_source': 'field-caught'}

Illumina NovaSeq X paired end sequencing

Illumina sequencing of sample accession SAMEA114806089 for study accession PRJEB59076. This submission includes reads tagged with the sequence CCTCTGCC.

{'gal': 'Natural History Museum', 'sex': 'NOT COLLECTED', 'tolid': 'icCriAspa2', 'habitat': 'NOT COLLECTED', 'lifestage': 'adult', 'broker name': 'COPO', 'common name': 'common asparagus beetle', 'external id': 'SAMEA114806089', 'sample name': '6560d2288a276eabc6e4d310', 'specimen_id': 'NHMUK014551787', 'collected_by': 'MICHAEL GEISER', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '6560d2288a276eabc6e4d310', 'gal_sample_id': 'NHMUK014551787', 'identified_by': 'MICHAEL GEISER', 'organism part': 'WHOLE ORGANISM', 'sample same as': 'SAMEA114805916', 'collection date': '2021-07-03', 'ena-last-update': '2023-12-06T13:23:17Z', 'scientific_name': 'Crioceris asparagi', 'barcoding center': 'NATURAL HISTORY MUSEUM', 'ena-first-public': '2023-12-06T13:23:17Z', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2023-12-06T13:23:17Z', 'insdc first public': '2023-12-06T13:23:17Z', 'collecting institution': 'NATURAL HISTORY MUSEUM', 'identifier_affiliation': 'NATURAL HISTORY MUSEUM', 'geographic location (latitude)': '51.41', 'geographic location (longitude)': '-0.06', 'sample collection device or method': 'Hand Picked', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'England|Penge|Winsford Gardens'}