Knautia arvensis

KnaArv

PacBio Iso-Seq libraries were constructed following the standard Iso-Seq library preparation protocol provided by Pacific Biosciences. Briefly, the process involved synthesizing full-length cDNA using the SMARTer PCR cDNA Synthesis Kit (Takara Bio), followed by PCR amplification and purification. The synthesized cDNA was then subjected to damage repair and terminal repair. SMRTbell adapters were ligated to the ends of the double-stranded cDNA molecules. The Sequel Binding and Internal Control Kit 3.0 (Pacific Biosciences) was used to bind primers and polymerase to the library for sequencing. Purification of the final library was performed using AMPure PB magnetic beads (Pacific Biosciences), and the libraries were loaded onto the PacBio Sequel II system for sequencing.

{'tissue': 'shoot', 'isolate': 'KnaArv01', 'lat_lon': '50.95121910 N 11.62093138 E', 'dev_stage': 'vegetative', 'geo_loc_name': 'Germany: Jena', 'biosamplemodel': 'Plant', 'collection_date': '2022-04'}