Zootermopsis

RNA-Seq of Zootermopsis angusticollis: transcriptomic signatures of termite queens

Examination of gene expression patterns in queen (abdomen tissue without gut). Tissue was preserved in RNAlater (Qiagen) and kept at 4C for 24 h and then at -20C until further processing. Total RNA was isolated from thawed tissue samples with the peqGOLD Tri Fast Kit using chloroform, nuclease-free glycogen (5 mg/ml), Ambion isopropanol, and 75% ethanol. DNA was digested by DNase I recombinant (Roche) and EDTA (Sigma-Aldrich). cDNA libraries were constructed using the transcriptome library construction TruSeq RNA Library Prep Kit v2 (Illumina). HiSeq Transcriptome Sequencing was done on an Illumina HiSeq Xten platform (150-bp paired-end reads).

{'age': '157 days post pairing', 'sex': 'female', 'tissue': 'Abdomen without gut', 'isolate': 'Primary queen', 'dev_stage': 'Adult', 'replicate': 'replicate 1', 'collected_by': 'Rebeca Rosengaus', 'geo_loc_name': 'USA:California', 'identified_by': 'Rebeca Rosengaus', 'biosamplemodel': 'Invertebrate', 'collection_date': '2020-07-27', 'biomaterial_provider': 'Rebeca Rosengaus'}

RNA-Seq of Zootermopsis angusticollis: transcriptomic signatures of termite queens

Examination of gene expression patterns in queen (abdomen tissue without gut). Tissue was preserved in RNAlater (Qiagen) and kept at 4C for 24 h and then at -20C until further processing. Total RNA was isolated from thawed tissue samples with the peqGOLD Tri Fast Kit using chloroform, nuclease-free glycogen (5 mg/ml), Ambion isopropanol, and 75% ethanol. DNA was digested by DNase I recombinant (Roche) and EDTA (Sigma-Aldrich). cDNA libraries were constructed using the transcriptome library construction TruSeq RNA Library Prep Kit v2 (Illumina). HiSeq Transcriptome Sequencing was done on an Illumina HiSeq Xten platform (150-bp paired-end reads).

{'age': '180 days post pairing', 'sex': 'female', 'tissue': 'Abdomen without gut', 'isolate': 'Primary queen', 'dev_stage': 'Adult', 'replicate': 'replicate 2', 'collected_by': 'Rebeca Rosengaus', 'geo_loc_name': 'USA:California', 'identified_by': 'Rebeca Rosengaus', 'biosamplemodel': 'Invertebrate', 'collection_date': '2020-07-21', 'biomaterial_provider': 'Rebeca Rosengaus'}

RNA-Seq of Zootermopsis angusticollis: transcriptomic signatures of termite queens

Examination of gene expression patterns in queen (head plus prothorax tissue). Tissue was preserved in RNAlater (Qiagen) and kept at 4C for 24 h and then at -20C until further processing. Total RNA was isolated from thawed tissue samples with the peqGOLD Tri Fast Kit using chloroform, nuclease-free glycogen (5 mg/ml), Ambion isopropanol, and 75% ethanol. DNA was digested by DNase I recombinant (Roche) and EDTA (Sigma-Aldrich). cDNA libraries were constructed using the transcriptome library construction TruSeq RNA Library Prep Kit v2 (Illumina). HiSeq Transcriptome Sequencing was done on an Illumina HiSeq Xten platform (150-bp paired-end reads).

{'age': '180 days post pairing', 'sex': 'female', 'tissue': 'Head+prothorax', 'isolate': 'Primary queen', 'dev_stage': 'Adult', 'replicate': 'replicate 1', 'collected_by': 'Rebeca Rosengaus', 'geo_loc_name': 'USA:California', 'identified_by': 'Rebeca Rosengaus', 'biosamplemodel': 'Invertebrate', 'collection_date': '2020-07-21', 'biomaterial_provider': 'Rebeca Rosengaus'}