Lyonetiidae

Harmaclona sp. transcriptome

Nucleic acids were extracted using Promega SV total RNA isolation mini-kits either with or without DNase digestion. Following DNase digestion, the RNA-only preps were subjected to poly-A selection and indexed library construction. The remaining extracts of total nucleic acids were used to produce cDNAs with low-input Clontech kits for either poly-dT priming (SMARTer Ultra Low input RNA kit Đv3, #634849) or universal priming (#634940). Following shearing to 200-bp size with a Covaris instrument, cDNA fragments were used for indexed library construction (Clontech kit #634947 for low input). Libraries were left unnormalized so as to favor highly expressed genes likely to be present in most species and all life stages.

{'tissue': 'whole specimen', 'isolate': 'DRD-05-0253', 'dev_stage': 'adult', 'geo_loc_name': 'USA: West Virginia', 'biosamplemodel': 'Invertebrate', 'collection_date': '2005-08-20', 'isolation_source': 'flew to light', 'specimen_voucher': 'DRD-05-0253'}