Hyalomma asiaticum

Transcriptome RNA-Seq of Hyalomma asiaticum: adult

Total RNA was used as input material for the RNA sample preparations. Briefly, mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR amplification, the PCR product was purified by AMPure XP beads, and the library was finally obtained.

{'host': 'rabbit', 'breed': 'H.asiaticum', 'tissue': 'body', 'isolate': 'Hyalomma asiaticum XinJiang strain', 'altitude': '337.48', 'dev_stage': 'adult', 'geo_loc_name': 'China', 'biosamplemodel': 'Invertebrate', 'collection_date': '2022-12'}

Transcriptome RNA-Seq of Hyalomma asiaticum: adult

Total RNA was used as input material for the RNA sample preparations. Briefly, mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR amplification, the PCR product was purified by AMPure XP beads, and the library was finally obtained.

{'host': 'rabbit', 'breed': 'H.asiaticum', 'tissue': 'body', 'isolate': 'Hyalomma asiaticum XinJiang strain', 'altitude': '337.49', 'dev_stage': 'adult', 'geo_loc_name': 'China', 'biosamplemodel': 'Invertebrate', 'collection_date': '2022-12'}

RNA-Seq of ticks: adult Hyalomma asiaticum

After tissue disruption and homogenization, RNA is extracted, and cDNA is reversed before RNA library construction.

{'host': 'Camelus', 'tissue': 'Insect body', 'isolate': 'not collected', 'geo_loc_name': 'China:Inner Mongolia', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-04-16', 'isolation_source': 'Alxa League'}