Taxillus

Loranthus seeds, cold treatment for 36 hours, replicate 3

Equal amount of total RNA (1 g) was used to construct the cDNA libraries for BGISEQ-500. In brief, mRNAs were enriched by magnetic oligo (dT) beads and fragmented into ~ 200 bp fragments, followed by the double strand cDNA library construction using random hexamer N6 primers. Next, the double strand cDNA libraries were end repaired by phosphate at the 5-end and sticky A to the 3-end. After the sequencing primers were ligated to each library and multiple libraries were pooled using the index technology, the pooled library was sequenced on the BGISEQ-500 RS platform with paired-end 150 (PE150) strategy at BGI-Shenzhen.

{'age': '1', 'temp': '0', 'tissue': 'Seeds', 'ecotype': 'China', 'isolate': 'T3', 'cultivar': 'Taxillus chinensis', 'dev_stage': '36h', 'treatment': 'Cold', 'geo_loc_name': 'China: Naning', 'biosamplemodel': 'Plant', 'isolation_source': 'Tree #3'}

Loranthus seeds, no cold treatment, replicate 1

Equal amount of total RNA (1 g) was used to construct the cDNA libraries for BGISEQ-500. In brief, mRNAs were enriched by magnetic oligo (dT) beads and fragmented into ~ 200 bp fragments, followed by the double strand cDNA library construction using random hexamer N6 primers. Next, the double strand cDNA libraries were end repaired by phosphate at the 5-end and sticky A to the 3-end. After the sequencing primers were ligated to each library and multiple libraries were pooled using the index technology, the pooled library was sequenced on the BGISEQ-500 RS platform with paired-end 150 (PE150) strategy at BGI-Shenzhen.

{'age': '1', 'temp': '0', 'tissue': 'Seeds', 'ecotype': 'China', 'isolate': 'T1', 'cultivar': 'Taxillus chinensis', 'dev_stage': '0', 'treatment': 'no treatment', 'geo_loc_name': 'China: Naning', 'biosamplemodel': 'Plant', 'isolation_source': 'Tree #1'}

Loranthus seeds, no cold treatment, replicate 2

Equal amount of total RNA (1 g) was used to construct the cDNA libraries for BGISEQ-500. In brief, mRNAs were enriched by magnetic oligo (dT) beads and fragmented into ~ 200 bp fragments, followed by the double strand cDNA library construction using random hexamer N6 primers. Next, the double strand cDNA libraries were end repaired by phosphate at the 5-end and sticky A to the 3-end. After the sequencing primers were ligated to each library and multiple libraries were pooled using the index technology, the pooled library was sequenced on the BGISEQ-500 RS platform with paired-end 150 (PE150) strategy at BGI-Shenzhen.

{'age': '1', 'temp': '0', 'tissue': 'Seeds', 'ecotype': 'China', 'isolate': 'T2', 'cultivar': 'Taxillus chinensis', 'dev_stage': '0', 'treatment': 'no treatment', 'geo_loc_name': 'China: Naning', 'biosamplemodel': 'Plant', 'isolation_source': 'Tree #2'}