Largidae

Transcriptome of Largus californicus: Sample LrspL1 - Largus californicus

"Construction of libraries and sequencing on the Illumina HiSeq2500 were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from one microgram of high quality total RNA. RNAseq libraries were constructed using the TruSeq Stranded RNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq2500 with TruSeq SBS sequencing kits version 3. The libraries were sequenced from both ends of the molecules to a total read length of 100nt from each end. The raw .bcl files were converted into demultiplexed fastq files with Casava 1.8.2 (Illumina)."

{'breed': 'wildtype', 'tissue': 'whole organism', 'isolate': 'LrspL1 - Largus californicus', 'lat_lon': '33.270208 N 116.419872 W', 'dev_stage': 'adult', 'collected_by': 'Christiane Weirauch', 'geo_loc_name': 'USA: California, San Diego County, Anza Borrego State Park', 'biosamplemodel': 'Invertebrate', 'collection_date': '2014-02-06', 'biomaterial_provider': 'Christiane Weirauch, Department of Entomology, University of California, 900 University Ave. Riverside, California 92521, USA'}

Transcriptome of Largus californicus: Sample LrspL2 - Largus californicus

"Construction of libraries and sequencing on the Illumina HiSeq2500 were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from one microgram of high quality total RNA. RNAseq libraries were constructed using the TruSeq Stranded RNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq2500 with TruSeq SBS sequencing kits version 3. The libraries were sequenced from both ends of the molecules to a total read length of 100nt from each end. The raw .bcl files were converted into demultiplexed fastq files with Casava 1.8.2 (Illumina)."

{'breed': 'wildtype', 'tissue': 'whole organism', 'isolate': 'LrspL2 - Largus californicus', 'lat_lon': '33.270208 N 116.419872 W', 'dev_stage': 'adult', 'collected_by': 'Christiane Weirauch', 'geo_loc_name': 'USA: California, San Diego County, Anza Borrego State Park', 'biosamplemodel': 'Invertebrate', 'collection_date': '2014-02-06', 'biomaterial_provider': 'Christiane Weirauch, Department of Entomology, University of California, 900 University Ave. Riverside, California 92521, USA'}