Cicadellidae

Transcriptome sequencing of Hishimonus sellatus

Total RNA was extracted from Hishimonus sellatus samples using Trizol reagent following the manufacturer's instructions. RNA integrity and quantity were verified using an Agilent 2100 Bioanalyzer. Poly(A) mRNA was enriched using oligo(dT) magnetic beads. The enriched mRNA was fragmented into short pieces. First-strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase, followed by second-strand cDNA synthesis using DNA Polymerase I and RNase H. The cDNA fragments were end-repaired, A-tailed, and ligated with sequencing adapters. After PCR amplification, the library was sequenced on an Illumina NovaSeq 6000 platform (PE150) to support gene prediction and annotation.

{'host': 'Morus alba', 'tissue': 'whole body', 'isolate': 'Hs-YZ-2024', 'geo_loc_name': 'China: Yizhou, Guangxi', 'biosamplemodel': 'Invertebrate', 'collection_date': '2025-11-12', 'isolation_source': 'mulberry orchard'}

Transcriptome sequencing of Hishimonus sellatus

Total RNA was extracted from Hishimonus sellatus samples using Trizol reagent following the manufacturer's instructions. RNA integrity and quantity were verified using an Agilent 2100 Bioanalyzer. Poly(A) mRNA was enriched using oligo(dT) magnetic beads. The enriched mRNA was fragmented into short pieces. First-strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase, followed by second-strand cDNA synthesis using DNA Polymerase I and RNase H. The cDNA fragments were end-repaired, A-tailed, and ligated with sequencing adapters. After PCR amplification, the library was sequenced on an Illumina NovaSeq 6000 platform (PE150) to support gene prediction and annotation.

{'host': 'Morus alba', 'tissue': 'whole body', 'isolate': 'Hs-YZ-2024', 'geo_loc_name': 'China: Yizhou, Guangxi', 'biosamplemodel': 'Invertebrate', 'collection_date': '2025-11-12', 'isolation_source': 'mulberry orchard'}

Illumina NovaSeq 6000 paired end sequencing

Illumina sequencing of sample accession SAMEA113425967 for study accession PRJEB108376. This submission includes reads tagged with the sequence CTTTCGAC.

{'gal': 'University of Oxford', 'sex': 'NOT COLLECTED', 'tolid': 'ihCicQuad5', 'habitat': 'Woodland', 'elevation': '150', 'lifestage': 'adult', 'broker name': 'COPO', 'external id': 'SAMEA113425967', 'sample name': '645b78f033cf76f22a67efbb', 'specimen_id': 'Ox003215', 'collected_by': 'Liam Crowley', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '645b78f033cf76f22a67efbb', 'gal_sample_id': 'Ox003215', 'identified_by': 'Liam Crowley', 'organism part': 'WHOLE ORGANISM', 'sample same as': 'SAMEA113425783', 'collection date': '2022-10-03', 'ena-last-update': '2023-05-11T10:58:09Z', 'scientific_name': 'Cicadula quadrinotata', 'barcoding center': 'NATURAL HISTORY MUSEUM', 'ena-first-public': '2023-05-11T10:58:09Z', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2023-05-11T10:58:09Z', 'insdc first public': '2023-05-11T10:58:09Z', 'collecting institution': 'University of Oxford', 'identifier_affiliation': 'University of Oxford', 'geographic location (latitude)': '51.764', 'geographic location (longitude)': '-1.337', 'sample collection device or method': 'Sweep net', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'Berkshire | Wytham Woods'}