Pterophoridae

Lantanophaga pusillidactyla transcriptome, GNV120027

RNA extracted from the thorax with the PROMEGA SV Total RNA Isolation System. library prep with ILLUMINA TruSeq RNA Sample Prep Kit v2. Library was pooled with 9 other samples and was run on a single lane of Illumina HISEQ2000. Data is single end length 100.

{'age': 'adult', 'sex': 'not determined', 'strain': '-', 'tissue': 'Whole body', 'geo_loc_name': 'USA: Florida, Gainesville', 'biosamplemodel': 'Model organism or animal', 'collection_date': '2012', 'biomaterial_provider': 'Kawahara'}

Acrolophus arcanellus transcriptome

Nucleic acids were extracted using Promega SV total RNA isolation mini-kits either with or without DNase digestion. Following DNase digestion, the RNA-only preps were subjected to poly-A selection and indexed library construction. The remaining extracts of total nucleic acids were used to produce cDNAs with low-input Clontech kits for either poly-dT priming (SMARTer Ultra Low input RNA kit Đv3, #634849) or universal priming (#634940). Following shearing to 200-bp size with a Covaris instrument, cDNA fragments were used for indexed library construction (Clontech kit #634947 for low input). Libraries were left unnormalized so as to favor highly expressed genes likely to be present in most species and all life stages.

{'tissue': 'whole specimen', 'isolate': 'NB-06-0144', 'dev_stage': 'adult', 'geo_loc_name': 'USA: California', 'biosamplemodel': 'Invertebrate', 'collection_date': '2006', 'specimen_voucher': 'NB-06-0144'}

Illumina NovaSeq 6000 paired end sequencing

Illumina sequencing of sample accession SAMEA10979454 for study accession PRJEB53158. This is part of an Illumina multiplexed sequencing run (45657_1). This submission includes reads tagged with the sequence GAGCGAAC.

{'gal': 'University of Oxford', 'sex': 'NOT COLLECTED', 'tolid': 'ilSteBipu3', 'habitat': 'Grassland', 'lifestage': 'adult', 'broker name': 'Collaborative Open Plant Omics broker account, Earlham Institute, Norwich', 'external id': 'SAMEA10979454', 'sample name': '618e9d3973e26544ea537b5c', 'specimen id': 'Ox001801', 'collected by': 'Will Langdon', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '618e9d3973e26544ea537b5c', 'gal_sample_id': 'Ox001801', 'identified by': 'Will Langdon', 'organism part': 'WHOLE ORGANISM', 'sample same as': 'SAMEA10979059', 'collection date': '2021-08-10', 'ena last update': '2021-11-15', 'barcoding center': 'NOT PROVIDED', 'ena first public': '2021-11-15', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2021-11-15T13:11:26Z', 'insdc center alias': 'EarlhamInstitute', 'insdc first public': '2021-11-15T13:11:26Z', 'collecting institution': 'University of Oxford', 'identifier_affiliation': 'University of Oxford', 'geographic location (latitude)': '51.77', 'geographic location (elevation)': '150', 'geographic location (longitude)': '-1.331', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'Berkshire | Wytham woods'}