Cryptocephalinae

RNA-Seq of Cryptocephalus sericeus: adult beetle

Total RNA was extracted using the innuPrep DNA/RNA Mini kit (Analytik Jena, Jena, Germany) following the manufacturer's instructions. Genomic DNA contamination was removed by DNAse treatment (TURBO DNase, Invitrogen, Carlsbad, CAL, USA) for 30 min at 37 C. Total RNA samples were further purified by using the RNeasy MinElute Clean up Kit (Qiagen, Hilden, Germany) following the manufacturers protocol, except for elution to 20 l volume. The integrity and quality of the RNA samples were determined using the RNA 6000 Nano LabChip kit on an Agilent 2100 Bioanalyzer (both Agilent Technologies, Santa Clara, CAL, USA) according to the manufacturers instructions. RNA-Seq was outsourced to the Max Planck Genome center (Cologne, Germany). There, poly(A)+ enriched RNA was fragmented to an average of 300-350 nucleotides and a TruSeq compatible, directional library was prepared for each sample. Libraries were tagged using dual indexing and were multiplexed on the same sequencing lane. Sequencing was carried out on a HiSeq3000 sequencing platform (Illumina, CA, USA) using paired-end (2 x 150 bp) reads.

{'tissue': 'whole insect', 'isolate': 'wild type individual', 'geo_loc_name': 'Italy', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018-05-15', 'isolation_source': 'field-caught'}

RNA-Seq of Pachybrachis hieroglyphicus: adult beetle

Total RNA was extracted using the innuPrep DNA/RNA Mini kit (Analytik Jena, Jena, Germany) following the manufacturer's instructions. Genomic DNA contamination was removed by DNAse treatment (TURBO DNase, Invitrogen, Carlsbad, CAL, USA) for 30 min at 37 C. Total RNA samples were further purified by using the RNeasy MinElute Clean up Kit (Qiagen, Hilden, Germany) following the manufacturers protocol, except for elution to 20 l volume. The integrity and quality of the RNA samples were determined using the RNA 6000 Nano LabChip kit on an Agilent 2100 Bioanalyzer (both Agilent Technologies, Santa Clara, CAL, USA) according to the manufacturers instructions. RNA-Seq was outsourced to the Max Planck Genome center (Cologne, Germany). There, poly(A)+ enriched RNA was fragmented to an average of 300-350 nucleotides and a TruSeq compatible, directional library was prepared for each sample. Libraries were tagged using dual indexing and were multiplexed on the same sequencing lane. Sequencing was carried out on a HiSeq3000 sequencing platform (Illumina, CA, USA) using paired-end (2 x 150 bp) reads.

{'tissue': 'whole insect', 'isolate': 'wild type individual', 'geo_loc_name': 'Italy', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018-05-15', 'isolation_source': 'field-caught'}

Illumina NovaSeq 6000 paired end sequencing

Illumina sequencing of sample accession SAMEA112221934 for study accession PRJEB54796. This is part of an Illumina multiplexed sequencing run (47846_4). This submission includes reads tagged with the sequence CAGGCTTT.

{'gal': 'Natural History Museum', 'sex': 'NOT PROVIDED', 'tolid': 'icCryMora3', 'habitat': 'NOT COLLECTED', 'lifestage': 'adult', 'broker name': 'Collaborative Open Plant Omics broker account, Earlham Institute, Norwich', 'external id': 'SAMEA112221934', 'sample name': '6393382c56e923203fff9f1c', 'specimen_id': 'NHMUK014037118', 'collected_by': 'OLGA SIVELL | DUNCAN SIVELL | RYAN MITCHELL', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '6393382c56e923203fff9f1c', 'gal_sample_id': 'NHMUK014037118', 'identified_by': 'RYAN MITCHELL', 'organism part': 'WHOLE ORGANISM', 'sample same as': 'SAMEA112221838', 'collection date': '2021-07-25', 'ena-last-update': '2022-12-12', 'barcoding center': 'NATURAL HISTORY MUSEUM', 'ena-first-public': '2022-12-12', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2022-12-12T08:45:29Z', 'insdc center alias': 'EarlhamInstitute', 'insdc first public': '2022-12-12T08:45:29Z', 'collecting institution': 'NATURAL HISTORY MUSEUM | NATURAL HISTORY MUSEUM | NATURAL HISTORY MUSEUM LONDON', 'identifier_affiliation': 'NATURAL HISTORY MUSEUM', 'geographic location (latitude)': '51.69', 'geographic location (longitude)': '-1.32', 'sample collection device or method': 'Hand Picked', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'England|Cothill Fen National Nature Reserve'}