Prodoxidae

Tegeticula yuccasella transcriptome

Total nucleic acids were extracted using Promega SV total RNA isolation mini-kits either with or without DNase digestion. Following DNase digestion, the RNA-only preps were subjected to poly-A selection and indexed library construction. The remaining extracts of total nucleic acids were used to produce cDNAs with low-input Clontech kits for either poly-dT priming (SMARTer Ultra Low input RNA kit Đv3, #634849) or universal priming (#634940). Following shearing to 200-bp size in a Covaris instrument, cDNA fragments were used for indexed library construction (Clontech kit #634947 for low input). Libraries were left unnormalized so as to favor highly expressed genes likely to be present in most species and all life stages.

{'tissue': 'abdomen', 'dev_stage': 'adult', 'geo_loc_name': 'USA: Maryland', 'biosamplemodel': 'Invertebrate', 'collection_date': '1991', 'isolation_source': 'raised from yucca pod', 'specimen_voucher': 'TPF-91-0074'}

RNA-Seq from Tegeticula yuccasella, heads

Construction of libraries and sequencing on Roche instruments were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign.

{'tissue': 'heads', 'isolate': 'wildtype', 'dev_stage': 'adult', 'geo_loc_name': 'USA', 'biosamplemodel': 'Invertebrate', 'collection_date': '2010', 'isolation_source': 'yucca'}