Incurvariidae

Phylotranscriptomics of Heliozelidae (Adeloidea, Lepidoptera)

RNA was extracted from specimens using the Nucleospin RNA XS (Macherey-Nagel) kit following the protocol for small samples, and RNA quality was checked using an Agilent 2200 TapeStation (Agilent Technologies). RNA libraries were prepared with the TruSeq mRNA library protocol for low inputs in half the standard reaction volume (25 uL) and quality checked using the TapeStation. Samples were multiplexed and sequenced on the Illumina NextSeq or MiSeq platform using paired end reads.

{'host': 'Acacia', 'tissue': 'whole body', 'collected_by': 'M Halsey', 'geo_loc_name': 'United Kingdom:Hampshire', 'biosamplemodel': 'Invertebrate', 'collection_date': '2016-05-25'}

Phylotranscriptomics of Heliozelidae (Adeloidea, Lepidoptera)

RNA was extracted from specimens using the Nucleospin RNA XS (Macherey-Nagel) kit following the protocol for small samples, and RNA quality was checked using an Agilent 2200 TapeStation (Agilent Technologies). RNA libraries were prepared with the TruSeq mRNA library protocol for low inputs in half the standard reaction volume (25 uL) and quality checked using the TapeStation. Samples were multiplexed and sequenced on the Illumina NextSeq or MiSeq platform using paired end reads.

{'host': 'Eucalyptus', 'tissue': 'whole body', 'collected_by': 'M Halsey', 'geo_loc_name': 'Australia:New South Wales', 'biosamplemodel': 'Invertebrate', 'collection_date': '2015-09-03'}

Illumina NovaSeq 6000 paired end sequencing

Illumina sequencing of sample accession SAMEA112975310 for study accession PRJEB55134. This is part of an Illumina multiplexed sequencing run (48340_1). This submission includes reads tagged with the sequence GGTATGCA.

{'gal': 'Natural History Museum', 'sex': 'male', 'tolid': 'ilIncMasc3', 'habitat': 'NOT COLLECTED', 'lifestage': 'adult', 'broker name': 'COPO', 'external id': 'SAMEA112975310', 'sample name': '642c40de88d00cdf3b11ea9d', 'specimen_id': 'NHMUK013697054', 'collected_by': 'DAVID LEES', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '642c40de88d00cdf3b11ea9d', 'gal_sample_id': 'NHMUK013697054', 'identified_by': 'DAVID LEES', 'organism part': 'HEAD|THORAX', 'collection date': '2022-04-08', 'ena-last-update': '2023-04-27T18:14:48Z', 'scientific_name': 'Incurvaria masculella', 'barcoding center': 'NATURAL HISTORY MUSEUM', 'ena-first-public': '2023-04-27T18:14:48Z', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2023-04-27T18:14:48Z', 'insdc first public': '2023-04-27T18:14:48Z', 'sample derived from': 'SAMEA112964167', 'collecting institution': 'NATURAL HISTORY MUSEUM', 'identifier_affiliation': 'NATURAL HISTORY MUSEUM', 'geographic location (latitude)': '51.68', 'geographic location (longitude)': '-0.81', 'sample collection device or method': 'Hand Picked', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'England|Bradenham'}