Choristoneura fumiferana

RNA-seq, adult tissues, male Choristoneura fumiferana, diapause strain

RNA extracted from adult female antennae, pheromone gland, brain, and reproductive tract

{'age': 'Newly emerged adults', 'sex': 'male', 'host': 'Abies balsamea', 'breed': 'Diapause strain', 'tissue': 'Antennae, reproductive tract, brain', 'isolate': 'Individuals from a lab colony', 'dev_stage': 'Adults', 'collected_by': 'Lisa Lumley', 'geo_loc_name': 'Canada:Ontario', 'biosamplemodel': 'Invertebrate', 'collection_date': '2012', 'env_broad_scale': 'Insect reared on artificial wheat-germ-based diet', 'biomaterial_provider': 'Great Lakes Forestry Centre, 1219 Queen Street, Sault Ste. Marie, ON, Canada, P6A 2E5'}

RNA-seq, adult tissues, female Choristoneura fumiferana, diapause strain

RNA extracted from adult male antennae, brain, and reproductive tract

{'age': 'Newly emerged adults', 'sex': 'female', 'host': 'Abies balsamea', 'breed': 'Diapause strain', 'tissue': 'Pheromone gland, antennae, reproductive tract, brain', 'isolate': 'Individuals from a lab colony', 'dev_stage': 'Adults', 'collected_by': 'Lisa Lumley', 'geo_loc_name': 'Canada:Ontario', 'biosamplemodel': 'Invertebrate', 'collection_date': '2012', 'env_broad_scale': 'Insect reared on artificial wheat-germ-based diet', 'biomaterial_provider': 'Great Lakes Forestry Centre, 1219 Queen Street, Sault Ste. Marie, ON, Canada, P6A 2E5'}

RNAseq library of Cfumiferana: male 6th instar larva

The transcriptome of a 6th instar non-diapause male larva was extracted with the TRIzol method (Rio et al., 2010) using Thermo Fishers TRIzol Reagent. Cell tissues were flash-frozen in liquid nitrogen and ground using a mortar and pestle; afterwards, the manufacturers recommended protocol was used. Total RNA was quantified with the Qubit RNA HS kit (Thermo Fisher), and purity was assessed with a NanoDrop 2000 spectrophotometer (Thermo Fisher). RNA was considered pure if A60/A280 and A260/A230 absorbance ratios were above 1.8. Total RNA was then sequenced natively (i.e. without the need for reverse transcription of ribosomal RNA depletion) with Oxfords Nanopores direct RNA sequencing procedure (SQK-RNA004) on a R9.4.1 flow cell (FLO-MIN106). Indeed, direct RNA sequencing adapters bind to poly-A tails in 3, thereby selectively binding to eukaryotic messenger RNA (SQK-RNA004).

{'age': '6th instar', 'sex': 'male', 'tissue': 'Whole insect', 'isolate': 'Glfc:IPQL:Cfum:non-diapause', 'dev_stage': 'larvae', 'geo_loc_name': 'Canada: Ontario', 'biosamplemodel': 'Invertebrate', 'collection_date': '2022', 'env_broad_scale': 'Insects reared in the lab under controlled environmental conditions, on wheat-germ-based artificial diet', 'isolation_source': 'GLFC SBW rearing', 'biomaterial_provider': 'Great Lakes Forestry Centre, Sault Ste. Marie, ON, Canada'}