Melampsora lini

RNA-Seq of Melampsora lini germinated urediniospores - replicate 3

Total RNA was isolated from up to 200 mg Linum usitatissium leaves inoculated by Melampsora lini at 8 days post inoculation using the RNeasy midi kit (Qiagen), according to the manufacturers instructions. Libraries were made and 50 bp single reads were generated in one lane with samples of Linum usitatissium leaves inoculated by Melampsora lini at 6 days post inoculation according to standard Illumina HiSeq protocols. The sequence reads were analysed according to Australian Genomic Research Facility quality control measures. Sequence quality was >95% bases above Q30. The reads were also screened for the presence of any Illumina adaptor/overrepresented sequences and cross-species contamination. The primary sequence data was generated using the Illumina consensus assessment of sequence and variation (CASAVA) pipeline (version 1.8.2).

{'host': 'Linum usitatissimum', 'isolate': 'CH5', 'sample_type': '6 hour germinated urediniospores', 'geo_loc_name': 'Australia: Canberra', 'biosamplemodel': 'Microbe, viral or environmental', 'isolation_source': 'leaves'}

RNA-Seq of Melampsora lini germinated urediniospores - replicate 1

Total RNA was isolated from up to 200 mg Linum usitatissium leaves inoculated by Melampsora lini at 8 days post inoculation using the RNeasy midi kit (Qiagen), according to the manufacturers instructions. Libraries were made and 50 bp single reads were generated in one lane with samples of Linum usitatissium leaves inoculated by Melampsora lini at 6 days post inoculation according to standard Illumina HiSeq protocols. The sequence reads were analysed according to Australian Genomic Research Facility quality control measures. Sequence quality was >95% bases above Q30. The reads were also screened for the presence of any Illumina adaptor/overrepresented sequences and cross-species contamination. The primary sequence data was generated using the Illumina consensus assessment of sequence and variation (CASAVA) pipeline (version 1.8.2).

{'host': 'Linum usitatissimum', 'isolate': 'CH5', 'sample_type': '6 hour germinated urediniospores', 'geo_loc_name': 'Australia: Canberra', 'biosamplemodel': 'Microbe, viral or environmental', 'isolation_source': 'leaves'}

RNA-Seq of Melampsora lini germinated urediniospores - replicate 2

Total RNA was isolated from up to 200 mg Linum usitatissium leaves inoculated by Melampsora lini at 8 days post inoculation using the RNeasy midi kit (Qiagen), according to the manufacturers instructions. Libraries were made and 50 bp single reads were generated in one lane with samples of Linum usitatissium leaves inoculated by Melampsora lini at 6 days post inoculation according to standard Illumina HiSeq protocols. The sequence reads were analysed according to Australian Genomic Research Facility quality control measures. Sequence quality was >95% bases above Q30. The reads were also screened for the presence of any Illumina adaptor/overrepresented sequences and cross-species contamination. The primary sequence data was generated using the Illumina consensus assessment of sequence and variation (CASAVA) pipeline (version 1.8.2).

{'host': 'Linum usitatissimum', 'isolate': 'CH5', 'sample_type': '6 hour germinated urediniospores', 'geo_loc_name': 'Australia: Canberra', 'biosamplemodel': 'Microbe, viral or environmental', 'isolation_source': 'leaves'}