Megachile

Illumina sequencing of RNAseq library from head of Megachile inimica: adult female

Total RNA was extracted with trizol + the zymo Directzol Magbead total RNA prep kit. We performed poly(A) selection during library prep via oligo dt with the NEB ultra II RNA library prep kit/modules.

{'age': 'Adult', 'sex': 'female', 'host': 'ex Coreopsis tinctoria (Asteraceae)', 'tissue': 'head', 'isolate': '=CONCATENATE(Q16, " ", V16)', 'lat_lon': '33.500393 N 90.835951 W', 'altitude': '36.36m', 'dev_stage': 'adult', 'collected_by': 'Kim T. Huntzinger and Daniel E. Warren', 'geo_loc_name': 'USA: MS, Washington Co., Hwy61, 2 mi N of Elizabeth', 'sample_notes': 'Megachile_inimica_SU2321_head', 'identified_by': 'K.T. Huntzinger', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-07-24', 'env_broad_scale': 'Mississippi Alluvial Plain', 'specimen_voucher': 'to be added', 'biomaterial_provider': 'Katherine Parys, Pollinator Health in Southern Crop Ecosystems Research Unit, US Department of Agriculture, Agricultural Research Service (USDA-ARS)'}

Illumina NovaSeq X paired end sequencing

Illumina sequencing of sample accession SAMEA112975378 for study accession PRJEB82540. This submission includes reads tagged with the sequence GGAGCAAG.

{'gal': 'Natural History Museum', 'sex': 'female', 'tolid': 'iyMegMari1', 'habitat': 'NOT COLLECTED', 'lifestage': 'adult', 'broker name': 'Collaborative Open Plant Omics broker account, Earlham Institute, Norwich', 'external id': 'SAMEA112975378', 'sample name': '642c40df88d00cdf3b11eae0', 'specimen_id': 'NHMUK015059182', 'collected_by': 'CLARE BOYES', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '642c40df88d00cdf3b11eae0', 'gal_sample_id': 'NHMUK015059182', 'identified_by': 'CLARE BOYES', 'organism part': 'WHOLE ORGANISM', 'sample same as': 'SAMEA112964216', 'collection date': '2022-07-05', 'ena-last-update': '2023-04-27', 'scientific_name': 'Megachile maritima', 'barcoding center': 'NATURAL HISTORY MUSEUM', 'ena-first-public': '2023-04-27', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2023-04-27T18:14:50Z', 'insdc center alias': 'EarlhamInstitute', 'insdc first public': '2023-04-27T18:14:50Z', 'collecting institution': 'DIPTERIST FORUM', 'identifier_affiliation': 'DIPTERIST FORUM', 'geographic location (latitude)': '52.73', 'geographic location (longitude)': '1.69', 'sample collection device or method': 'Hand Picked', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'England| Winterton Dunes'}

Megachile posti Illumina RNA-Seq data

{'gal': 'SciLifeLab', 'sex': 'female', 'tolid': 'iyMegPost2', 'habitat': 'Open pine forest with underlying dry scrubland, including Noaea mucronata', 'lifestage': 'adult', 'broker name': 'COPO', 'sample name': '67123299309589ca2f206e62', 'specimen_id': 'ERGA AP 4894 00556', 'bio_material': 'NOT_APPLICABLE', 'collected_by': 'RÉMI SANTERRE', 'project name': 'ERGA', 'submitter id': '67123299309589ca2f206e62', 'gal_sample_id': 'NOT PROVIDED', 'identified_by': 'RÉMI SANTERRE', 'organism part': 'WHOLE ORGANISM', 'proxy voucher': 'MNCN Ent 395801', 'collection date': '2024-06-13', 'scientific_name': 'Megachile posti', 'specimen_voucher': 'NOT_APPLICABLE', 'insdc center name': 'EarlhamInstitute', 'proxy biomaterial': 'MNCN-ADN-203169', 'collector orcid id': '0009-0001-2128-6568', 'sample coordinator': 'ANNA PAPADOPOULOU', 'collecting institution': 'Cyprus University of Technology', 'identifier_affiliation': 'Cyprus University of Technology', 'sample collection method': 'Collected using an insect net, placed in a sterile tube and then in a dry shipper with liquid nitrogen', 'sample coordinator orcid id': '0000-0002-4656-4894', 'geographic location (latitude)': '35.008915', 'sample coordinator affiliation': 'UNIVERSITY OF CYPRUS', 'geographic location (longitude)': '33.955724', 'geographic location (country and/or sea)': 'Cyprus', 'geographic location (region and locality)': 'Famagusta |Sotira'}