Cornu aspersum

RNA-Seq of Cornu aspersum mucus-secreting tissue

Snails provided by Peconic Escargot in February 2020 were sacrificed on-site via freezing in a dry ice-ethanol mixture. Whole snails were stored in Invitrogen RNAlater (Thermo Fisher, AM7021) and frozen at 80 C until used. Total RNA was extracted using a Qiagen RNeasy Micro kit (Qiagen, 74004) according to manufacturers instructions, from 6 individual tissue slices from different snails. The integrity of total RNA was confirmed using nanodrop and Agilent 2100 BioAnalyzer analysis. Total RNA was used as a template to perform polyA enriched first strand cDNA synthesis using the HiSeq RNA sample preparation kit for Illumina Sequencing (Illumina Inc., CA) following manufacturers instructions. The cDNA libraries were sequenced using Illumina HiSeq 1000 technology using a paired end flow cell and 80 x 2 cycle sequencing.

{'breed': 'Cornu aspersum food-grade snails', 'tissue': 'Epithelium', 'replicate': 'Biological Replicate 6', 'geo_loc_name': 'USA: Cutchogue, NY', 'biosamplemodel': 'Invertebrate', 'collection_date': '2020-02', 'isolation_source': 'Farm-raised snails, Peconic escargot'}

Illumina NovaSeq 6000 paired end sequencing

Illumina sequencing of sample accession SAMEA9312975 for study accession PRJEB75282. This submission includes reads tagged with the sequence CATGAATC.

{'gal': 'Sanger Institute', 'sex': 'hermaphrodite and monoecious', 'tolid': 'xgCorAspe11', 'habitat': 'Rough grass', 'lifestage': 'adult', 'broker name': 'COPO', 'common name': 'brown garden snail', 'external id': 'SAMEA9312975', 'sample name': '603d0e497b00d800011081d5', 'specimen id': 'SAN0001344', 'collected by': 'ANGUS DAVISON', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '603d0e497b00d800011081d5', 'gal_sample_id': 'SAN0001344', 'identified by': 'ANGUS DAVISON', 'organism part': '**OTHER SOMATIC ANIMAL TISSUE**', 'collection date': '2019-12-05', 'ena-last-update': '2021-07-01T13:47:11Z', 'scientific_name': 'Cornu aspersum', 'ena-first-public': '2021-07-01T13:47:11Z', 'specimen voucher': 'NOT COLLECTED', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2021-07-01T13:47:11Z', 'insdc first public': '2021-07-01T13:47:11Z', 'sample derived from': 'SAMEA9312911', 'collecting institution': 'University of Nottingham', 'identifier_affiliation': 'University of Nottingham', 'geographic location (latitude)': '52.914711', 'geographic location (longitude)': '-1.189386', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'Nottingham | Beeston | Beeston Rylands | NG9 1NG | Public footpath by Casuals rugby club'}