Choreutidae

Hemerophila diva transcriptome

{'label': 'Cor5', 'isolate': 'JR-08-020', 'dev_stage': 'adult', 'geo_loc_name': 'USA: Florida', 'collection_date': '2008', 'specimen_voucher': 'JR-08-020'}

Opogona thiadelia transcriptome

Nucleic acids were extracted using Promega SV total RNA isolation mini-kits either with or without DNase digestion. Following DNase digestion, the RNA-only preps were subjected to poly-A selection and indexed library construction. The remaining extracts of total nucleic acids were used to produce cDNAs with low-input Clontech kits for either poly-dT priming (SMARTer Ultra Low input RNA kit Đv3, #634849) or universal priming (#634940). Following shearing to 200-bp size with a Covaris instrument, cDNA fragments were used for indexed library construction (Clontech kit #634947 for low input). Libraries were left unnormalized so as to favor highly expressed genes likely to be present in most species and all life stages.

{'tissue': 'whole specimen', 'isolate': 'JR144-SL3', 'dev_stage': 'adult', 'geo_loc_name': 'Slovakia', 'biosamplemodel': 'Invertebrate', 'collection_date': '2006-07-06', 'isolation_source': 'flew to light', 'specimen_voucher': 'JR144-SL3'}

Compsoctena sp. transcriptome

Nucleic acids were extracted using Promega SV total RNA isolation mini-kits either with or without DNase digestion. Following DNase digestion, the RNA-only preps were subjected to poly-A selection and indexed library construction. The remaining extracts of total nucleic acids were used to produce cDNAs with low-input Clontech kits for either poly-dT priming (SMARTer Ultra Low input RNA kit Đv3, #634849) or universal priming (#634940). Following shearing to 200-bp size with a Covaris instrument, cDNA fragments were used for indexed library construction (Clontech kit #634947 for low input). Libraries were left unnormalized so as to favor highly expressed genes likely to be present in most species and all life stages.

{'tissue': 'whole specimen', 'isolate': 'JR120-LS3', 'dev_stage': 'adult', 'geo_loc_name': 'Costa Rica', 'biosamplemodel': 'Invertebrate', 'collection_date': '2006-09-01', 'isolation_source': 'flew to light', 'specimen_voucher': 'JR120-LS3'}