Dactylopius coccus

Dactylopius transcriptome

Half a milligram of frozen adult female Dactylopius coccus, obtained from Lanzarote Spain, were ground into a fine powder with a mortar and pestle under liquid nitrogen. Total RNA was subsequently extracted using the RNeasy mini kit (Qiagen) according to manufacturer’s instructions. Polyadenylated RNA was converted into cDNA with an oligo-dT primer and a reverse transcriptase (RT˛ Easy First Strand Kit, Qiagen). The cDNA samples were sequenced with a total yield of 5GB/sample (corresponding to 55 million 90-bp reads) by BGI-Shenzhen, China using 90-bp paired-end Illumina sequencing technology

{'sex': 'female', 'tissue': 'Whole insect', 'dev_stage': 'Adult', 'biosamplemodel': 'Model organism or animal'}

Dactylopius coccus Illumina RNA-Seq data

{'gal': 'SciLifeLab', 'sex': 'female', 'tolid': 'ihDacCocc7', 'habitat': 'Exotic forest', 'lifestage': 'adult', 'broker name': 'COPO', 'sample name': '670b67ec91c50845d5ed1306', 'specimen_id': 'ERGA PR 7524 0894', 'collected_by': 'Paula Riccardi | Dora Aguín-Pombo | Hugo Miguel Silva | Gonçalo Gomes | Luena Marques | Emily Hartop', 'project name': 'ERGA', 'submitter id': '670b67ec91c50845d5ed1306', 'gal_sample_id': 'NOT PROVIDED', 'identified_by': 'DORA AGUIN-POMBO', 'organism part': 'WHOLE ORGANISM', 'proxy voucher': 'UMACI 7524 0907', 'sample same as': 'SAMEA116283125', 'collection date': '2024-08-27', 'scientific_name': 'Dactylopius coccus', 'specimen_voucher': 'NOT PROVIDED', 'insdc center name': 'EarlhamInstitute', 'proxy biomaterial': 'MNCN-ADN 204306', 'collector orcid id': '0000-0003-4850-7524 | 0000-0002-4471-7105 | 0009-0005-7396-6273 | 0009-0002-7388-7864 | 0009-0009-4235-6033 | 0000-0002-8598-9844', 'sample coordinator': 'PAULA RICCARDI', 'collecting institution': 'Museum für Naturkunde Berlin | University of Madeira | University of Madeira | University of Madeira | University of Madeira | Norwegian University of Science and Technology', 'identifier_affiliation': 'University of Madeira', 'voucher institution url': 'https://ror.org/0442zbe52', 'sample collection method': 'Sweeping', 'sample coordinator orcid id': '0000-0003-4850-7524', 'geographic location (latitude)': '32.646775', 'sample coordinator affiliation': 'Museum für Naturkunde Berlin', 'geographic location (longitude)': '-16.862051', 'geographic location (country and/or sea)': 'Portugal', 'geographic location (region and locality)': 'Madeira Island | Santa Cruz | Cancela'}