Scaphytopius

Transcriptome of Scaphytopius frontalis

Construction of libraries and sequencing on the Illumina HiSeq4000 were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from one microgram of high quality total RNA. RNAseq libraries were constructed using the TruSeq Stranded RNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq4000 with HiSeq 4000 sequencing kit version 1. The libraries were sequenced from both ends of the molecules to a total read length of either 100nt or 150nt from each end. The raw .bcl files were converted into demultiplexed fastq files with bcl2fastq v2.17.1.14 Conversion Software (Illumina).

{'breed': 'wildtype', 'tissue': 'whole insect', 'collected_by': 'C. Dietrich', 'geo_loc_name': 'USA: Illinois, Ford County, Rt. 45 at Pit Rd. S. Paxton', 'biosamplemodel': 'Invertebrate', 'collection_date': '2016-08-24', 'isolation_source': 'wild-caught'}