Agrostemma githago

RNA-SEQ of Agrostemma githago L.

Plant tissue was ground in liquid nitrogen and stored at -80C before RNA extraction. RNA was extracted from all samples following steps 2-7 from the protocol of Wang et. al.(2012) and continued from step 2 in the RNAeasy plant kit (Qiagen, Inc., Valencia, CA, USA) manual. Quality and concentration of the extracted RNA was measured using NanoDrop (Thermo Fisher Scientific, Inc., Wilmington, DE, USA). RNA-seq library preparation and sequencing of the samples were performed at SciLifeLabs Facility in Stockholm, Sweden (https://www.scilifelab.se/). 2x100bp paired end read libraries were prepared using TruSeq Stranded Total RNA LT Sample Prep Kit - Set A (with Ribo-Zero Plant). (Serial number RS-122-2401.) using a custom protocol on an Agilent Bravo pipetting robot (Agilent, Santa Clara, USA).

{'tissue': 'roots, stem, leaves, developing buds, flowers, fruits', 'isolate': '17748', 'dev_stage': 'Fully mature', 'treatment': 'RNAlater', 'sample_type': 'Whole organism', 'geo_loc_name': 'Sweden: Hisingen', 'biosamplemodel': 'Plant', 'collection_date': '2011'}

tRNA-seq of total cellular RNA from Agrostemma githago

AlkB treatment and YAMAT-Seq

{'age': '4 weeks', 'tissue': 'Leaf and stem', 'isolate': 'Replicate 3', 'replicate': 'Agrostemma total cellular 3', 'geo_loc_name': 'USA:Colorado', 'biosamplemodel': 'Plant', 'biomaterial_provider': 'Kew Gardens Millennium Seed Bank'}

PacBio IsoSeq of Agrostemma githago: leaf and flower RNA pool

PacBio IsoSeq

{'age': '4 weeks', 'tissue': 'Leaf and Flower', 'isolate': 'Kew0053084', 'geo_loc_name': 'Russia', 'biosamplemodel': 'Plant'}

Illumina NovaSeq X paired end sequencing

Illumina sequencing of sample accession SAMEA9335348 for study accession PRJEB62047. This submission includes reads tagged with the sequence GACTCCAC.

{'gal': 'Royal Botanic Gardens Kew', 'sex': 'hermaphrodite and monoecious', 'tolid': 'dcAgrGith1', 'habitat': 'nursery greenhouse', 'lifestage': 'vegetative structure', 'broker name': 'COPO', 'common name': 'corn cockle', 'external id': 'SAMEA9335348', 'sample name': '60dd902f6206260001b53a0b', 'specimen id': 'KDTOL10241', 'collected by': 'SAHR MIAN', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '60dd902f6206260001b53a0b', 'gal_sample_id': 'KDTOL10241', 'identified by': 'Sahr Mian', 'organism part': 'LEAF', 'collection date': '2021-06-02', 'ena-last-update': '2021-07-05T11:44:26Z', 'scientific_name': 'Agrostemma githago', 'ena-first-public': '2021-07-05T11:44:26Z', 'specimen voucher': 'SM32', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2021-07-05T11:44:26Z', 'insdc first public': '2021-07-05T11:44:26Z', 'sample derived from': 'SAMEA9335250', 'collecting institution': 'ROYAL BOTANIC GARDENS KEW', 'identifier_affiliation': 'ROYAL BOTANIC GARDENS KEW', 'geographic location (latitude)': '51.48319', 'geographic location (elevation)': '8m', 'geographic location (longitude)': '-0.29304', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'SURREY | KEW GARDENS'}