Uroleucon

Transcriptome of Uroleucon ambrosiae: Sample Uramb - Uroleucon ambrosiae

"Construction of libraries and sequencing on the Illumina HiSeq2500 were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from one microgram of high quality total RNA. RNAseq libraries were constructed using the TruSeq Stranded RNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq2500 with TruSeq SBS sequencing kits version 3. The libraries were sequenced from both ends of the molecules to a total read length of 100nt from each end. The raw .bcl files were converted into demultiplexed fastq files with Casava 1.8.2 (Illumina)."

{'breed': 'wildtype', 'tissue': 'whole organism', 'dev_stage': 'adult', 'collected_by': 'Allison K. Hansen', 'geo_loc_name': 'USA: lab culture at University of Illinois, Champaign-Urbana', 'biosamplemodel': 'Invertebrate', 'collection_date': '2014-04-03', 'biomaterial_provider': 'Allison K. Hansen, Dept. Entomology, University of Illinois, 505 S. Goodwin Ave, Urbana, Illinois 61801, USA'}

RNAseq of Uroleucon eupatoricolens adults

Total RNA with Ribo-Zero Plus and NEBNext rRNA Depletion Kit

{'host': 'Eutrochium purpureum', 'breed': 'NA', 'tissue': 'body', 'isolate': 'A119,A120', 'lat_lon': '35.8155 N 83.2478 W', 'dev_stage': 'Adult', 'collected_by': 'P. Rozo-Lopez', 'geo_loc_name': 'USA: TN, Cosby', 'identified_by': 'P. Rozo-Lopez', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-06-05', 'isolation_source': 'aphid'}

RNAseq of Uroleucon spp adults

Total RNA with Ribo-Zero Plus and NEBNext rRNA Depletion Kit

{'host': 'Eutrochium purpureum', 'breed': 'NA', 'tissue': 'body', 'isolate': 'A090,A095,A103,A122', 'lat_lon': '35.8155 N 83.2478 W', 'dev_stage': 'Adult', 'collected_by': 'P. Rozo-Lopez', 'geo_loc_name': 'USA: TN, Cosby', 'identified_by': 'P. Rozo-Lopez', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-05-23', 'isolation_source': 'aphid'}