Pachygrontha

Transcriptome of Pachygrontha sp. KJ-2015

"Construction of libraries and sequencing on the Illumina HiSeq2500 were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from one microgram of high quality total RNA. RNAseq libraries were constructed using the TruSeq Stranded RNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq2500 with TruSeq SBS sequencing kits version 4. The libraries were sequenced from both ends of the molecules to a total read length of 100nt from each end. The raw .bcl files were converted into demultiplexed fastq files with Casava 1.8.2 (Illumina)."

{'breed': 'wildtype', 'tissue': 'whole insect', 'lat_lon': '27.25395 S 152.10706 E', 'collected_by': 'Christopher H. Dietrich and Kevin P. Johnson', 'geo_loc_name': "Australia: QLD, Crow's Nest N.P.", 'biosamplemodel': 'Invertebrate', 'collection_date': '2015-02-22', 'isolation_source': 'wild-caught', 'specimen_voucher': 'AU8'}