Aphelenchoides avenae

Single-nematode RNA-seq of second instar larvae stage of Aphelenchus avenae

The low-input mRNA from each of the individual embryos was then subjected to reverse transcription into cDNA using a sensitive highly-multiplexed single-cell RNA-seq (Smart-seq2) protocol.

{'host': 'Botrytis cinerea', 'breed': 'HZAU-1', 'tissue': 'mix stage', 'isolate': 'BMB_Lab', 'geo_loc_name': 'China: Wuhan', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018-09-10', 'isolation_source': 'A small piece of about 0.5 cm square is cut from the pure cultured Botrytis cinerea and then transferred to fresh PDA medium. After culturing it in the dark at 28 degrees for 3 days, when the hyphae grows but has not yet covered the culture dish, the collected A. avenae nematodes are transferred to the culture dish. After culturing for another 4-6 weeks, the nematodes collected on the lid of the culture dish can be used for experiments.'}

Single-nematode RNA-seq of second instar larvae stage of Aphelenchus avenae

The low-input mRNA from each of the individual embryos was then subjected to reverse transcription into cDNA using a sensitive highly-multiplexed single-cell RNA-seq (Smart-seq2) protocol.

{'host': 'Botrytis cinerea', 'breed': 'HZAU-1', 'tissue': 'mix stage', 'isolate': 'BMB_Lab', 'geo_loc_name': 'China: Wuhan', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018-09-10', 'isolation_source': 'A small piece of about 0.5 cm square is cut from the pure cultured Botrytis cinerea and then transferred to fresh PDA medium. After culturing it in the dark at 28 degrees for 3 days, when the hyphae grows but has not yet covered the culture dish, the collected A. avenae nematodes are transferred to the culture dish. After culturing for another 4-6 weeks, the nematodes collected on the lid of the culture dish can be used for experiments.'}

ISO-seq of the mix stage of Aphelenchus avenae

In order to analyze the gene transcription of A. avenae and annotate its genome more comprehensively, we performed ISO-seq on nematodes of mixed ages.

{'host': 'Botrytis cinerea', 'breed': 'Aa01', 'tissue': 'mix stage', 'isolate': 'Aphelenchus avenae is a fungus-feeding nematode with a mouth needle that can also parasitize strawberries.', 'geo_loc_name': 'China: Wuhan, Hubei', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018-09-10'}