Xyleborus glabratus

RNA-seq of Xyleborus glabratus: adult female whole body, technical replicate 4

Total RNA was isolated with TRIzol reagent (Life Technologies) and purified with a RNeasy MiniElute kit (QIAGEN) from 5-7 adult females of Xyleborus glabratus, according to the manufacturers instructions. The RNA integrity was assessed by chip-based capillary gel electrophoresis using an Agilent 2100 Bioanalyzer system (Agilent Technologies). The RNA concentration was determined by absorbance at 260 nm using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific). A total of 500 ng RNA was used as input material to prepare each of the four RNA-seq libraries used in this study. We used the RNA-seq method based on poly(A) selection, which enriches for eukaryotic mRNA and other polyadenylated RNA molecules. The TruSeq RNA Sample Preparation Kit (Illumina) was used for this purpose adding specific indexes to each of the sequenced samples. All libraries were sequenced on a NextSeq 550 platform (Illumina) using a 2 x 150 bp paired-end sequencing protocol, were 150 bases from each side of DNA strands are sequenced.

{'host': 'Persea americana', 'tissue': 'whole organism', 'geo_loc_name': 'USA: Florida, Homestead.', 'biosamplemodel': 'Invertebrate'}

RNA-seq of Xyleborus glabratus: adult female whole body, technical replicate 3

Total RNA was isolated with TRIzol reagent (Life Technologies) and purified with a RNeasy MiniElute kit (QIAGEN) from 5-7 adult females of Xyleborus glabratus, according to the manufacturers instructions. The RNA integrity was assessed by chip-based capillary gel electrophoresis using an Agilent 2100 Bioanalyzer system (Agilent Technologies). The RNA concentration was determined by absorbance at 260 nm using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific). A total of 500 ng RNA was used as input material to prepare each of the four RNA-seq libraries used in this study. We used the RNA-seq method based on poly(A) selection, which enriches for eukaryotic mRNA and other polyadenylated RNA molecules. The TruSeq RNA Sample Preparation Kit (Illumina) was used for this purpose adding specific indexes to each of the sequenced samples. All libraries were sequenced on a NextSeq 550 platform (Illumina) using a 2 x 150 bp paired-end sequencing protocol, were 150 bases from each side of DNA strands are sequenced.

{'host': 'Persea americana', 'tissue': 'whole organism', 'geo_loc_name': 'USA: Florida, Homestead.', 'biosamplemodel': 'Invertebrate'}

RNA-seq of Xyleborus glabratus: adult female whole body, technical replicate 2

Total RNA was isolated with TRIzol reagent (Life Technologies) and purified with a RNeasy MiniElute kit (QIAGEN) from 5-7 adult females of Xyleborus glabratus, according to the manufacturers instructions. The RNA integrity was assessed by chip-based capillary gel electrophoresis using an Agilent 2100 Bioanalyzer system (Agilent Technologies). The RNA concentration was determined by absorbance at 260 nm using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific). A total of 500 ng RNA was used as input material to prepare each of the four RNA-seq libraries used in this study. We used the RNA-seq method based on poly(A) selection, which enriches for eukaryotic mRNA and other polyadenylated RNA molecules. The TruSeq RNA Sample Preparation Kit (Illumina) was used for this purpose adding specific indexes to each of the sequenced samples. All libraries were sequenced on a NextSeq 550 platform (Illumina) using a 2 x 150 bp paired-end sequencing protocol, were 150 bases from each side of DNA strands are sequenced.

{'host': 'Persea americana', 'tissue': 'whole organism', 'geo_loc_name': 'USA: Florida, Homestead.', 'biosamplemodel': 'Invertebrate'}