Jacobaea vulgaris

RNA-Seq of Jacobaea vulgaris: pooled shoots and roots of 59 individuals

Total RNA was extracted with the NucleoSpin RNA Plant-Macherey-Nagel kit, mRNA was selected using oligo-dT Dynabeads. Then, RNA were fragmented to generate double stranded cDNA followed by duplex-specific thermostable nuclease treatment to remove abundant library molecules. Sequencing was performed on a HiSeq2500 platform.

{'tissue': 'shoot&root', 'isolate': 'including 59 individuals', 'cultivar': 'Yangan Chen', 'dev_stage': 'vegetative', 'biosamplemodel': 'Plant'}

RNA-Seq of Jacobaea vulgaris: control of methyl jasmonate treated shoot cultures

Total RNA was extracted with the NucleoSpin RNA Plant-Macherey-Nagel kit, mRNA was selected using oligo-dT Dynabeads. Then, RNA were fragmented to generate double stranded cDNA to be sequenced on a HiSeq2500 platform.

{'tissue': 'shoot (tissue culture)', 'isolate': 'Control of methyl jasmonate treated Jacobaea vulgaris', 'cultivar': 'Yangan Chen', 'dev_stage': 'vegetative', 'geo_loc_name': 'Netherlands', 'biosamplemodel': 'Plant'}

transcriptome of Jacobaea vulgaris subsp. dunensis

Asteraceae phylogeny based on nex-generation sequencing

{'tissue': 'young leaves', 'sub_species': 'dunensis', 'geo_loc_name': 'Netherlands', 'biosamplemodel': 'Plant', 'specimen_voucher': 'FUS: Hong Ma HM1069'}

Illumina NovaSeq 6000 paired end sequencing

Illumina sequencing of sample accession SAMEA112689632 for study accession PRJEB79869. This submission includes reads tagged with the sequence CGATTGCA.

{'gal': 'Royal Botanic Garden Edinburgh', 'sex': 'hermaphrodite and monoecious', 'tolid': 'daJacVulg1', 'habitat': 'in sandy short grassland', 'lifestage': 'sporophyte', 'broker name': 'COPO', 'external id': 'SAMEA112689632', 'sample name': '63f8c3b18e0cf9658dc67436', 'specimen_id': 'EDTOL04470', 'collected_by': 'MARKUS RUHSAM', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '63f8c3b18e0cf9658dc67436', 'gal_sample_id': 'EDTOL04470', 'identified_by': 'MARKUS RUHSAM', 'organism part': 'WHOLE PLANT', 'collection date': '2022-08-03', 'ena-last-update': '2023-02-27T09:34:01Z', 'scientific_name': 'Jacobaea vulgaris', 'barcoding center': 'ROYAL BOTANIC GARDEN EDINBURGH', 'ena-first-public': '2023-02-27T09:34:01Z', 'specimen_voucher': 'NOT PROVIDED', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2023-02-27T09:34:01Z', 'insdc first public': '2023-02-27T09:34:01Z', 'sample derived from': 'SAMEA112689537', 'collecting institution': 'ROYAL BOTANIC GARDEN EDINBURGH', 'identifier_affiliation': 'ROYAL BOTANIC GARDEN EDINBURGH', 'voucher institution url': 'https://ror.org/0349vqz63', 'geographic location (latitude)': '56.024475', 'geographic location (longitude)': '-2.8574527', 'sample collection device or method': 'picked leaves were cut into FluidX tubes', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'Aberlady'}