Tetriginae

RNA-Seq of Ergatettix serrifemora: adult female whole body

Total RNA was extracted from whole adult specimens using TRIzol Reagent (Invitrogen, USA) following the manufacturer's protocol. RNA integrity was assessed using a NanoDrop 2000 (Thermo Fisher Scientific, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, USA); only samples with RNA Integrity Number (RIN) >7.0 were used. RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, USA) according to the supplier's instructions. The libraries were quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific) and their size distribution assessed with an Agilent 2100 Bioanalyzer. Sequencing was performed using the Illumina HiSeq 2000 platform with a paired-end 150 bp configuration.

{'tissue': 'Whole-body', 'lat_lon': '25.0047 N 108.9085 E', 'geo_loc_name': 'China:Luocheng Mulao Autonomous County', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-08-20'}

RNA-Seq of Ergatettix serrifemora: adult female whole body

Total RNA was extracted from whole adult specimens using TRIzol Reagent (Invitrogen, USA) following the manufacturer's protocol. RNA integrity was assessed using a NanoDrop 2000 (Thermo Fisher Scientific, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, USA); only samples with RNA Integrity Number (RIN) >7.0 were used. RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, USA) according to the supplier's instructions. The libraries were quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific) and their size distribution assessed with an Agilent 2100 Bioanalyzer. Sequencing was performed using the Illumina HiSeq 2000 platform with a paired-end 150 bp configuration.

{'tissue': 'Whole-body', 'lat_lon': '25.0047 N 108.9088 E', 'geo_loc_name': 'China:Luocheng Mulao Autonomous County', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-08-20'}

RNA-Seq of Ergatettix serrifemora: adult female whole body

Total RNA was extracted from whole adult specimens using TRIzol Reagent (Invitrogen, USA) following the manufacturer's protocol. RNA integrity was assessed using a NanoDrop 2000 (Thermo Fisher Scientific, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, USA); only samples with RNA Integrity Number (RIN) >7.0 were used. RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, USA) according to the supplier's instructions. The libraries were quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific) and their size distribution assessed with an Agilent 2100 Bioanalyzer. Sequencing was performed using the Illumina HiSeq 2000 platform with a paired-end 150 bp configuration.

{'tissue': 'Whole-body', 'lat_lon': '25.0047 N 108.9087 E', 'geo_loc_name': 'China:Luocheng Mulao Autonomous County', 'biosamplemodel': 'Invertebrate', 'collection_date': '2023-08-20'}