Melanoplinae

RNAseq of Melanoplus sanguinipes: adult male whole insect

Total RNA was extracted by homogenizing in TRIzol reagent according to the manufacturer's protocol (Invitrogen). Total RNA was resuspended in sterile DEPC-treated H2O and quantified by measuring absorbance at 260 and 280 nm using a Nanodrop spectrophotometer (Fisher-Thermo). RNA was run through the Micro poly-A purist column (Fisher-Thermo) to select for polyadenylated transcripts. The quality of the RNA was assessed using an RNA 6000 Nano Kit and a Bioanalyzer 2100 instrument (Agilent). Illumina sequencing libraries were synthesized and sequencing performed at the National Research Council, Saskatoon, Canada

{'tissue': 'Adult male - whole insect', 'isolate': 'AAFC Saskatoon colony', 'dev_stage': 'Adult', 'geo_loc_name': 'Canada: Deslisle', 'biosamplemodel': 'Invertebrate', 'collection_date': '2011', 'isolation_source': 'AAFC Saskatoon colony, started in 1958 with insects collected in Deslisle SK.'}

RNAseq of Melanoplus sanguinipes: hyaline gland from mated males

Total RNA was extracted by homogenizing in TRIzol reagent according to the manufacturer's protocol (Invitrogen). Total RNA was resuspended in sterile DEPC-treated H2O and quantified by measuring absorbance at 260 and 280 nm using a Nanodrop spectrophotometer (Fisher-Thermo). RNA was run through the Micro poly-A purist column (Fisher-Thermo) to select for polyadenylated transcripts. The quality of the RNA was assessed using an RNA 6000 Nano Kit and a Bioanalyzer 2100 instrument (Agilent). 454 sequencing libraries were synthesized and sequencing performed at the National Research Council, Saskatoon, Canada

{'tissue': 'pooled Hyaline Glands from mated male insects', 'isolate': 'AAFC Saskatoon colony', 'dev_stage': 'Adult', 'geo_loc_name': 'Canada: Deslisle', 'biosamplemodel': 'Invertebrate', 'collection_date': '2011', 'isolation_source': 'AAFC Saskatoon colony, started in 1958 with insects collected in Deslisle SK.'}

RNAseq of Melanoplus sanguinipes: hyaline gland from virgin males

Total RNA was extracted by homogenizing in TRIzol reagent according to the manufacturer's protocol (Invitrogen). Total RNA was resuspended in sterile DEPC-treated H2O and quantified by measuring absorbance at 260 and 280 nm using a Nanodrop spectrophotometer (Fisher-Thermo). RNA was run through the Micro poly-A purist column (Fisher-Thermo) to select for polyadenylated transcripts. The quality of the RNA was assessed using an RNA 6000 Nano Kit and a Bioanalyzer 2100 instrument (Agilent). 454 sequencing libraries were synthesized and sequencing performed at the National Research Council, Saskatoon, Canada

{'tissue': 'pooled Hyaline Glands from virgin male insects', 'isolate': 'AAFC Saskatoon colony', 'dev_stage': 'Adult', 'geo_loc_name': 'Canada: Deslisle', 'biosamplemodel': 'Invertebrate', 'collection_date': '2011', 'isolation_source': 'AAFC Saskatoon colony, started in 1958 with insects collected in Deslisle SK.'}