Sphecodes

RNA seq of Sphecodes autumnalis female Dufour's

Library Preparation following kit instructions: NEBNext Ultra II RNA Library Prep Kit for Illumina

{'age': '376 Meters', 'depth': '42.55466, -76.23009', 'tissue': "Dufour's gland_rep1", 'altitude': 'Callum Kingwell', 'dev_stage': 'female', 'collected_by': 'Silas Bossert', 'geo_loc_name': 'USA: field near Essex Steel building, Cortland, NY.', 'biosamplemodel': 'Invertebrate', 'collection_date': '2016-09-01', 'env_broad_scale': 'USNMENT01135713', 'host_tissue_sampled': "Dissected Dufour's gland flash-frozen fresh in liquid nitrogen", 'biomaterial_provider': 'Adult'}

Illumina HiSeq 4000 paired end sequencing

Illumina sequencing of sample accession SAMEA7746812 for study accession PRJEB45661. This is part of an Illumina multiplexed sequencing run (44268_6). This submission includes reads tagged with the sequence GGGTACTT.

{'gal': 'University of Oxford', 'sex': 'male', 'tolid': 'iySphMoni1', 'habitat': 'Woodland', 'lifestage': 'adult', 'broker name': 'COPO', 'external id': 'SAMEA7746812', 'sample name': '5fdb7c6e150c500001125f05', 'specimen id': 'Ox000852', 'collected by': 'Steven Falk', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '5fdb7c6e150c500001125f05', 'gal_sample_id': 'Ox000852c', 'identified by': 'Steven Falk', 'organism part': 'ABDOMEN', 'collection date': '2020-08-20', 'ena-last-update': '2021-07-02T15:21:37Z', 'scientific_name': 'Sphecodes monilicornis', 'ena-first-public': '2020-12-22T15:19:21Z', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2021-07-02T15:21:37Z', 'insdc first public': '2020-12-22T15:19:21Z', 'sample derived from': 'SAMEA7746755', 'collecting institution': 'University of Oxford', 'identifier_affiliation': 'University of Oxford', 'geographic location (latitude)': '51.769', 'geographic location (elevation)': '150', 'geographic location (longitude)': '-1.339', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'Berkshire | Wytham woods'}

Illumina NovaSeq 6000 paired end sequencing

Illumina sequencing of sample accession SAMEA110451823 for study accession PRJEB55971. This is part of an Illumina multiplexed sequencing run (47503_3). This submission includes reads tagged with the sequence CACTTTGC.

{'gal': 'University of Oxford', 'sex': 'female', 'tolid': 'iySphEphi2', 'habitat': 'Woodland', 'elevation': '150', 'lifestage': 'adult', 'broker name': 'Collaborative Open Plant Omics broker account, Earlham Institute, Norwich', 'external id': 'SAMEA110451823', 'sample name': '62d9228586eb2e0eb5ebfb03', 'specimen_id': 'Ox002174', 'collected_by': 'Steven Falk', 'insdc status': 'public', 'project name': 'DTOL', 'submitter id': '62d9228586eb2e0eb5ebfb03', 'gal_sample_id': 'Ox002174', 'identified_by': 'Steven Falk', 'organism part': 'WHOLE ORGANISM', 'sample same as': 'SAMEA110451616', 'collection date': '2022-05-19', 'ena-last-update': '2022-08-01', 'barcoding center': 'NATURAL HISTORY MUSEUM', 'ena-first-public': '2022-08-01', 'insdc center name': 'EarlhamInstitute', 'insdc last update': '2022-08-01T16:51:58Z', 'insdc center alias': 'EarlhamInstitute', 'insdc first public': '2022-08-01T16:51:59Z', 'collecting institution': 'University of Oxford', 'identifier_affiliation': 'University of Oxford', 'geographic location (latitude)': '51.769', 'geographic location (longitude)': '-1.328', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'Berkshire | Wytham Woods'}