Dictyopharidae

Transcriptome of Dictyopharini sp.: Sample DispD1 - Dictyopharini sp.

"Construction of libraries and sequencing on the Illumina HiSeq2500 were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from one microgram of high quality total RNA. RNAseq libraries were constructed using the TruSeq Stranded RNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq2500 with TruSeq SBS sequencing kits version 3. The libraries were sequenced from both ends of the molecules to a total read length of 100nt from each end. The raw .bcl files were converted into demultiplexed fastq files with Casava 1.8.2 (Illumina)."

{'breed': 'wildtype', 'tissue': 'whole organism', 'lat_lon': '39.8913 S 72.7943 W', 'dev_stage': 'adult', 'collected_by': 'Christopher H. Dietrich', 'geo_loc_name': 'Chile: Los Lagos, Rt. 5 2km S Los Lagos', 'biosamplemodel': 'Invertebrate', 'collection_date': '2014-01-17', 'biomaterial_provider': 'Christopher H. Dietrich, Illinois Natural History Survey, University of Illinois, 1816 South Oak Street, Champaign, Illinois 61820, USA'}

Transcriptome of Phylloscelis atra: Sample Phatr - Phylloscelis atra

"Construction of libraries and sequencing on the Illumina HiSeq2500 were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from one microgram of high quality total RNA. RNAseq libraries were constructed using the TruSeq Stranded RNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq2500 with TruSeq SBS sequencing kits version 3. The libraries were sequenced from both ends of the molecules to a total read length of 100nt from each end. The raw .bcl files were converted into demultiplexed fastq files with Casava 1.8.2 (Illumina)."

{'breed': 'wildtype', 'tissue': 'whole organism', 'lat_lon': '40.1044 N 88.2114 W', 'dev_stage': 'adult', 'collected_by': 'Christopher H. Dietrich', 'geo_loc_name': 'USA: Illinois, Champaign County, Urbana', 'biosamplemodel': 'Invertebrate', 'collection_date': '2013-07-29', 'biomaterial_provider': 'Christopher H. Dietrich, Illinois Natural History Survey, University of Illinois, 1816 South Oak Street, Champaign, Illinois 61820, USA'}

RNA-Seq of Pentastiridius sp.adult

cDNA library was gained by mRNA enrichment and reverse transcription PCR

{'age': 'adult', 'breed': 'wildtype', 'tissue': 'whole body', 'geo_loc_name': 'China:Beijing Shunyi District, Zhangzhen', 'biosamplemodel': 'Invertebrate', 'collection_date': '2014-06-06'}