Depressariidae

Nucleic acids were extracted using Promega SV total RNA isolation mini-kits either with or without DNase digestion. Following DNase digestion, the RNA-only preps were subjected to poly-A selection and indexed library construction. The remaining extracts of total nucleic acids were used to produce cDNAs with low-input Clontech kits for either poly-dT priming (SMARTer Ultra Low input RNA kit Đv3, #634849) or universal priming (#634940). Following shearing to 200-bp size with a Covaris instrument, cDNA fragments were used for indexed library construction (Clontech kit #634947 for low input). Libraries were left unnormalized so as to favor highly expressed genes likely to be present in most species and all life stages.

{'tissue': 'whole specimen', 'isolate': 'JCS-06-0112', 'dev_stage': 'adult', 'geo_loc_name': 'South Korea', 'biosamplemodel': 'Invertebrate', 'collection_date': '2004-06-18', 'isolation_source': 'flew to light', 'specimen_voucher': 'JCS-06-0112'}

Illumina NovaSeq X paired end sequencing

Illumina sequencing of sample accession SAMEA114645024 for study accession PRJEB47461. This submission includes reads tagged with the sequence CCGGCTGG.

{'gal': 'University of Oxford', 'sex': 'NOT COLLECTED', 'tolid': 'ilAgoSubp2', 'habitat': 'Woodland', 'elevation': '150', 'lifestage': 'adult', 'broker name': 'COPO', 'sample name': '655b386ffbcb208dd5510a1a', 'specimen_id': 'Ox003318', 'collected_by': 'Liam Crowley', 'project name': 'DTOL', 'submitter id': '655b386ffbcb208dd5510a1a', 'gal_sample_id': 'Ox003318', 'identified_by': 'Liam Crowley', 'organism part': 'WHOLE ORGANISM', 'sample same as': 'SAMEA114644424', 'collection date': '2022-11-01', 'scientific_name': 'Agonopterix subpropinquella', 'barcoding center': 'NATURAL HISTORY MUSEUM', 'insdc center name': 'EarlhamInstitute', 'collecting institution': 'University of Oxford', 'identifier_affiliation': 'University of Oxford', 'sample collection method': 'Light trap', 'geographic location (latitude)': '51.772', 'geographic location (longitude)': '-1.338', 'geographic location (country and/or sea)': 'United Kingdom', 'geographic location (region and locality)': 'Berkshire | Wytham Woods'}

Illumina NovaSeq X paired end sequencing

Illumina sequencing of sample accession SAMEA117574344 for study accession PRJEB105627. This submission includes reads tagged with the sequence TTCCTGGG.

{'gal': 'University of Oulu', 'sex': 'female', 'tolid': 'ilExaCini2', 'habitat': 'Not recorded', 'symbiont': 'N', 'lifestage': 'adult', 'sample name': '103864', 'specimen_id': 'OULUPSY000368', 'collected_by': 'SAMUEL LAM', 'project name': 'PSYCHE', 'submitter id': '103864', 'gal_sample_id': 'ZMUO.086722', 'identified_by': 'SAMUEL LAM', 'organism part': 'ABDOMEN', 'collection date': '2024-05-09', 'scientific_name': 'Exaeretia ciniflonella', 'specimen_voucher': 'NOT PROVIDED', 'insdc center name': 'Tree of Life Programme', 'sample derived from': 'SAMEA117574256', 'collecting institution': 'University of Oulu', 'identifier_affiliation': 'University of Oulu', 'geographic location (latitude)': '64.9666216', 'geographic location (longitude)': '25.7548536', 'geographic location (country and/or sea)': 'Finland', 'geographic location (region and locality)': 'Oulu | Pilpasuo'}