Lonchaeidae

RNA-Seq of Neosilba glaberrima

Extractions were performed with the RNeasy kit (Qiagen, Valencia, CA). Library preparation used the NEBNext (New England Biosciences, Ipswich, MA, USA) Ultra RNA Library Prep Kit for Illumina kit. RNA was bound to Agencourt AMPure XP Beads (Beckman Coulter, Inc., Brea, CA, USA) on a magnetic plate. A reverse transcription reaction is performed, followed by a PCR enrichment, yielding a size-selected non-directional cDNA library to be sequenced, multiplexed, as paired-end reads on an Illumina system (Illumina, San Diego, CA, USA)

{'sex': 'female', 'tissue': 'whole body', 'isolate': 'neosi', 'lat_lon': '9.4732 N 83.5918 W', 'altitude': '1515m', 'dev_stage': 'adult', 'collected_by': 'B. Wiegmann', 'geo_loc_name': 'Costa Rica: Limon Prov., San Gerardo de Rivas, Perez Zeledon, Talamanca Nature Reserve near Chirripo National Park, on window of lodge', 'identified_by': 'Keith Bayless', 'biosamplemodel': 'Invertebrate', 'collection_date': '2014-04-11', 'isolation_source': 'wild', 'specimen_voucher': 'NCSU_neosi'}