Piezodorus guildinii

RNA-seq Piezodorus guildinii

Total RNA was purified from all nymphal stages (1st - 5th) and adults (male and female) using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer instructions. RNA integrity was determined with a Bioanalyzer 2100 (Agilent technologies). Equal RNA quantity of every stage was pooled to a 1 g sample used for cDNA library preparation and Illumina sequencing, conducted by Macrogen, Inc. (Seoul, South Korea). TruSeq stranded mRNA libraries were generated with a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and sequencing was performed with Illumina HiSeq 2500 platform with a coverage of 75G (1 lane), 150bp paired ends reads

{'breed': '-', 'tissue': 'Hole body', 'isolate': 'Pool RNA from 1st, 2nd, 3rd, 4th, 5th instar and adult (male and female)', 'geo_loc_name': 'Uruguay:Colonia', 'biosamplemodel': 'Invertebrate', 'collection_date': '2019', 'isolation_source': 'Alfalfa crops'}

Transcriptome of Piezodorus guildinii nymphs

Total RNA was extracted from whole bodies of ten first instar nymphs of each species using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), following manufacturers instructions. Total RNA was quantitated on a Nanodrop 2000c spectrophotometer (Thermo Scientific, Wilmington, USA) and its quality assessed with 1% (w/v) agarose gel electrophoresis. Immediately after extraction, 2 g of total RNA of each sample was lyophilized and shipped to Novogene (Davis, USA) for sequencing on an Illumina NovaSeq platform. The construction of cDNA libraries was performed from RNA samples for Illumina paired end (PE) sequencing following the Illumina protocol. The sequencing strategy was PE150, which gives 150bp read from each end to make a 300bp sequenced fragment.

{'age': 'first instar nymphs', 'tissue': 'whole body', 'isolate': 'field-collected insects', 'geo_loc_name': 'Argentina', 'biosamplemodel': 'Invertebrate', 'collection_date': '2022-10'}