Tobamovirus

Cucumber green mottle mosaic virus

1. DNA digest: total RNA was treated with DNase I which degraded double-stranded and single stranded DNA presenting in RNA samples.; 2. rRNA removal: using the Ribo-off rRNA Depletion Kit to remove ribosomal RNA.; 3. RNA fragmentation.; 4. cDNA Synthesis: First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis with dUTP instead of dTTP.; 5. End repair, add A and adaptor ligation.; 6. PCR amplification: The PCR reaction system and program are configured and set up to amplify the product.; 7. Library quality control.; 8. Cyclization: Denature the PCR product by heat and the DNA is cyclized by splint oligo and DNA ligase.; 9. DNA nanoball (DNB) synthesis.; 10. Sequencing on DNBSEQ platform.

{'isolate': 'Iraq-1', 'collected_by': 'Athraa A. Hadi', 'geo_loc_name': 'Iraq', 'biosamplemodel': 'Pathogen.env', 'collection_date': '2025', 'isolation_source': 'Thrips insects'}

Metatranscriptomics of thrips

DNBSEQ Short-read library preparation 1. DNA fragmentation 2. Size selection 3. End repair and "A" tailing 4. Adapter ligation 5. Product size selection 6. PCR reaction 7. Library QC

{'isolate': 'Iraq-1', 'collected_by': 'Athraa A. Hadi', 'geo_loc_name': 'Iraq', 'biosamplemodel': 'Pathogen.env', 'collection_date': '2025', 'isolation_source': 'Thrips insects'}

To_Jo_Sh

cDNA library

{'host': 'tomato', 'isolate': 'To_Jo_Sh', 'lab_host': 'manure', 'geo_loc_name': 'Jordan', 'biosamplemodel': 'Viral', 'collection_date': '2023-05-15', 'isolation_source': 'manure'}